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Blood collection
Blood is collected in plastic bag systems
with anticoagulant & preservative
Whole blood can be stored at 4 oC for up to
5 weeks
Whole blood contains many components
Wasteful to give whole blood if only red
cells are needed
Blood Components
Human blood consists of
plasma, in which cells are
suspended
The plasma also contains
other specialised substances,
which are important for blood
clot formation (e.g. clotting
factors)
Whole blood can be separated
at the blood bank into various
components
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BLOOD COMPONENTS
Blood separated into different parts:
1.
2.
3.
4.
5.
6.
7.
Whole blood
Red cells
Granulocytes
Platelets
Plasma
Fractionated
products
(Fresh) frozen
plasma (FFP)
F Vlla
Cryoprecipitate
Stored
Plasma
Immunoglobulins
F Vlll
Albumin
F lX
Whole Blood
One unit contains
450 ml of blood
& 63 ml of anticoagulant-preservative
Anticoagulant-Preservative Contents
Red cells
5 weeks in CPD-A have a mean recovery 70%
Plasma
K+, H+ pH
Levels of coagulation factors V & VIII decrease
Blood Components
Refers to a product separated from a
single unit of whole blood
The term plasma derivative indicates a
blood product separated from a large
volume of pooled plasma by a process
called fractionation
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Blood components
Oxygen carrying components
Red cell concentrates
(RCC)
Leukocyte poor blood
Frozen-thawed red cells
Platelet products
Platelet rich plasma (PRP)
Platelet concentrates (PC)
Plasma products
Plasma Derivatives
Coagulation Factor concentrates
Factor VIII concentrates
Factor IX complex concentrates &
others
Oncotic agents
Albumin
Plasma protein fraction (PPF)
Hepatitis B Ig (HBIG)
Varicella-zoster Ig (VZIG)
Rh Ig (RhIG)
Tetanus Ig (TIG)
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Red cell
concentrate
Total Volume
500 ml
300 ml
Volume of red
cells
200 ml
200 ml
Volume of
plasma
300 ml
100 ml
Hematocrit
40 %
70 %
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Glucose solutions
can cause clumping
Buffy coat
Red cells
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Fluorinated hydrocarbons
Readily dissolve oxygen
Poor soluble in plasma
Side effects:
Hypotension
DIC
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B- Platelet Products
Platelet Rich Plasma (PRP)
Gentle centrifugation of whole blood
Supernatant transferred to the 2nd
bag
Platelet Concentrates
Prepared from PRP by a 2nd
centrifugation
Removal of all but 50 ml of plasma
Contain approx. 6X1010 platelets
60 80% Plts present in whole blood
unit
Remain 5 days
Longer at 22oC with continuous
agitation
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B- Platelet Products
Contamination by WBCs &
RBCs is usually small
But there is enough to induce
alloimmunization
Plt concentrates from Rh +ve
should not be administered to
Rh ve women
Storage at 22oC, therefore care
to prevent contamination
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C- Plasma Products
Plt poor plasma can be separated into a
number of products
Fresh frozen plasma
Frozen plasma
Cryoprecipitate
Stored plasma
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3- Cryoprecipitate
Produced from freshly separated plasma by
freezing at -70oC followed by thawing at 4oC
Flocculent precipitate is rich in factor VIII,
fibrinogen and fibronectin
Once thawed, mixture is centrifuged to sediment
the cryoprecipitate & all but 5 to 10 ml of
supernatant plasma is removed
Contains 250 mg fibrinogen
80 clotting units of factor VIII
Stored at -30oC for 12 months
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3- Cryoprecipitate
Increase of 2% of factor VIII level for each
bag of cryoprecipitate infused
Supernatant plasma removed is called
stored plasma
Must be used within 5 weeks if stored at 4oC
Lasts for 2 years at -30oC
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4- Stored plasma
Plasma separated from whole blood after 24 hours of
storage at 4oC
Can also be derived from cryoprecipitate production
Contain reduced levels of labile coagulation factors V VIII
& fibrinogen
It is indicated for patients requiring volume expansion or
protein replacement when labile clotting factors are not
required
Plasma products do not require crossmatch prior to use
but should be ABO compatible
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Plasma Derivatives
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pooled plasma
Pooling of many units increases the risk of
viral transmission to the recipient
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Plasma Derivatives
Plasma Derivatives
Preparation avaliable
Factor VIII concentrates
Coagulation Factors
Factor IX concentrates
Anti-thrombin III
Albumin
Albumin
Plasma protein fraction
Non-specific immune serum globulin (ISG)
Rh immune globulin (RhIG)
Immune globulins
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Cryoprecipitate
Factor VIII
concentrates
4oC
Short period RT
Risk of
Hepatitis
Low
High
Treatment of
hemophilia A
Yes
Yes
Treatment of
vW disease
Yes
no
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Factor IX Concentrate
For the treatment Factor IX deficiency or
Hemophilia B (Christmas Disease).
Have been used to treat patients with
acquired inhibitors of factor VIII
Have factor VIII bypassing activity
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Factor IX
Factor IX concentrate
Factor X
Stored plasma
Factor XI
Stored plasma
Factor XIII
Stored plasma
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2- Oncotic Agents
Albumin: volume expansion
Other colloids are available for blood
volume expansion
Dextran
Gelatin
Hydroxyethyl starch
Polyvinylpyrrolidone
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Albumin
Albumin is prepared by ethanol
fractionation of pooled plasma
Available in 5% and 25% concentrations.
Have physiological sodium content
No risk of hepatitis, sterilized during
preparation
No coagulation factors or blood group Abs
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Albumin
Used for treatment of hypovolaemia and
hypoalbuminaemia (result from abnormal
synthesis, increased metabolism or loss)
It maintains capillary osmotic pressure
Carrier protein for drugs, hormones,
enzymes & metabolites
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3- Immune Globulins
Contains immune IgG antibodies,
prepared from pools of plasma.
For disease prophylaxis, hepatitis A,
measles, varicella and rubella.
For the treatment of hypogammaglobulinemia and agammaglobulinemia.
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Primarily IgG Ab
Prevention of some viral diseases
Hypogammaglobulinemia
Congenital immune deficiency
Given by IM injection (aggregates of IgG)
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