WHAT IS A COLORIMETRIC?

Colorimetry is a method of
chemical analysis are based on
the achievement of the amount of
color similarity between the
sample solution with the standard
solution by using a light source
polikromatis.

Tools : Colorimeter

 This method is based on the absorption of

visible light and other radiation energy by a
solution.
This method can be applied to the
determination of the dye component or
components are not colored, but by using the
appropriate reagent dye can produce a
colored compound which is a function of the
content of its components.

 If you have reached the similarity of color

means the number of dye molecules
absorbing light passed on both sides are
same, and this has been used as the basis
of calculation.
Examples are made nitrite solution colored
with Sulfanilamide reagent and N- (1naphthyl) -etilendiamin.?
PS: The amount of radiation absorbed is
proportional to the concentration of
absorbent substances in solution.

Colorimetric divided into two methods, namely:

a) Visual colorimetric
Using eye as a detector.
Visual colorimetric method is a conventional
method and is rarely used because it is not
accurate. This is because the eye only as a
detector to see the similarity in color, not
as a measure of the intensity of absorption.
b) Photometry:

Using a photocell detector.

1. Series Standard Method (Method Nesler):

In this method, made a series of standard
solutions in the tubes of the same size with
the same kind anyway.
Visual colorimetric analysis methods there are four kinds:

2. Balance Method
In this method is done by comparing the
sample solution with a standard solution
which is based on a standard solution of
varied thickness.
This method is divided into three, namely:
1. - Cylinder System Hehner
2. - Bajerum Comparator
3. - Dubosq colorimetri

3. dilution method: using a standard

substance and a burette containing blank.
Standard concentration is diluted with
blank until there is similarity in color.
4. The standard method of synthesis: the
investigated substance obtained by the
addition of a number of standard
components against a blank solution until
there is similarity in color.

Terms Determining Concentration With Visual

Colorimetric Method:
A. High Solution Constant (Constant depht
Methods)
Divided into two methods:
1. Nessler Tube
In this method, used several cylindrical
reaction tube. Each tube is filled with a
standard solution with a concentration of
high scalable and varies with the same
solution.

Nessler
Tube

2. Bajerum Comparator
In this tool, to achieve the color similarity
between the sample solution with the
standard solution is done by shifting the
solution

Bajerum
Comparator

B. High contrast solution (Variable Depth

Methods)
Divided into two methods:
1. Hehner Tubes
Hehner the form of a pair of cylindrical tube
with a tap to remove the solution from the
cylinder that the color of the solution is
more concentrated, so height is changed, in
order to have the same color on both
cylinders.

Herner
Tube

2. Dubosq Colorimeter
The principle of this tools is cooperation
with the colorimeter Nessler tube. A
color comparison tool equipped with
binoculars.
In this tool color similarity is obtained by
regulating the level of ballast
(plunger), so that the solution in the
vessel height changed to obtain the
same color intensity on spiltfield.

binoculars

Dubosq
Colorimete
r

spacer

Standard
footage

Terms colorimetric method is the solution should be

colorless. If the solution is colorless then performed
first with the addition of reagents pengomplekan
dyes.
While the staining conditions, among others:
- Color formed should be stable
- Be selective staining reaction
- Solution should be transparent
- High sensitivity
- Re-high accuracy
- Color formed to be a function of concentration.

T
H
A
N
K
S