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CLINICAL HEMATOLOGY
Vet . Heba Yasser
BLOOD
BLOOD
BLOOD COMPOSITION
The cellular
portion
is assessed in
the clinical
hematology
laboratory
The liquid
portion
evaluated in the
clinical
biochemistry
laboratory.
PHYSIOLOGICAL CONSIDERATIONS IN
INTERPRETATION OF BLOOD VALUES
1. Emotional stress: facing different environment in
the veterinary clinic, Blood should be collected when
the animal is at rest.
PHYSIOLOGICAL CONSIDERATIONS
IN INTERPRETATION OF BLOOD
VALUES
3. Diet: Samples shouldn't be collected soon or
Emotional
stress:
Exercise:
Prolonged
exercise:
Diet: after
meals
Starvation:
Physiological function
Altered test
Hyperglycemia
Lecucytosis
Lymphocytosis in
cats
Hypoxia
Release of blood
induce spleenic cells into
contraction
circulation
increases activity of muscular
enzymes
lipemic
response
Epinephrine
Drugs:
Glucocorticoids
PCV
TLC
CK, AST, LDH
Physiological
Drugs:
exogenous
insulin
Antibiotics :
Cephalosporins
Age, sex and
breed
response
decreases
serum glucose
function
Altered test
Blood glucose
test and serum
potassium
increased
creatinine
concentration
Significantly influences some of the blood values.
2. Anticoagulants
SYRINGES : PRECAUTIONS
The
Using
4.
Lancets
veins.
Occlude the vein using digital pressure or
tourniquet.
Stretch the skin across the vein.
Insert the needle, while the bevel is directed
upward.
Apply gentile traction or suction of blood to
avoid collapse of the vein.
Release the digital pressure, or remove the
tourniquet.
Gently withdraw the blood by the plungers.
Jugular vein
Sampling from
coccogyeal artery
in cow
Jugular vein in
lamb
Sampling in mice
ANTICOAGULANTS
Hematological examination requires blood in
liquid form.
Immediately after withdrawal; blood must be
thoroughly mixed with the anticoagulant to
prevent clotting.
The common used Anticoagulants are: Heparin,
EDTA, Sodium fluoride, Sodium citrate and
double oxalates.
Mode of
action
Heparine
EDTA
Sod.Citrate
Sod.flouride
Double
oxalate
Dose
Recommended
uses
Advantages
Disadvantages
CAUSES OF HEMOLYSIS
A) Before withdrawal:
Using wet syringe or needle.
Using needle of small gauge.
B) During withdrawal:
Rapid withdrawal of the blood.
Moving the needle inside the vein.
Placing the blood into the vacuum tubes too
quickly.
C) After withdrawal:
o
LIPEMIA
Lipemia falsely decrease serum sodium and
potassium measurements by flame photometry.
Lipemia falsely increase plasma proteins
measurement by refracto- metry.
Lipemia causes false decrease in cholesterol in
cats.