Microbiology laboratoric

test

Gram Stain
Material
solutions of crystal violet,
Grams iodine (2 g
potassium iodide in 300 ml
distilled water plus 1 g
iodine crystals), 95%
ethanol and/or
isopropanol-acetone
mixture (3:1 v/v), and
safranin
Bismark brown stain (for
color-blind students)
clean glass slides
inoculating loop
Bunsen burner
bibulous paper

microscope
lens paper and lens cleaner
immersion oil
Hyphomonas
(Hyphomicrobium)
neptunium (ATCC 15444)
grown in marine broth (Difco)
slide warmer
staining rack
Bacto Gram Stain Reagents
from Difco for the three-step
Gram stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Examination the preparation under microscope ( x100) for confirmation

Microscopic result for Gram

Stain