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Bacterial Genetics
Important Point:
Bacterial Genetics
Types of Mutations
Base Substitution
Point mutation = single base is substituted.
Missense mutation = base change changes single
amino acid to different amino acid.
Nonsense mutation = base change changes single
amino acid to stop codon.
Null or Knockout mutation = mutation that totally
inactivates a gene.
Deletion or insertion mutation = change in number of
bases making up a gene.
Frameshift mutation = insertion or deletion of
something other than multiples of three bases.
Frameshifts typically radically change downstream
codons, generating stop codons, and typically
knocking out gene function.
Reversion mutation = mutated change back to that of
wild type.
Rates of Mutation
Indirect Selection:
Penicillin
Enrichment
Indirect Selection:
Isolation of ts
Mutants
This is one example of isolation of mutants carrying
conditionally lethal mutations found in essential genes.
DNA-Mediated Transformation
DNA-Mediated Transformation
Artificial Competence
by Electroporation
Competence
denotes the
ability to take
up DNA naked
from the
environment.
Most bacteria
are not naturally
competent but
many can be
made artificially
so.
Artificially
induced
competence is
very important
to gene cloning.
Generalized Transduction
Bacteriophages
are viruses that
only infect (and
can kill)
bacteria.
Generalized Transduction
Self-Transmissible R Plasmid
Note
multiple
resistance
genes.
Note in
particular that
DNA is cut at
palindromic
regions.