Laporan Kemajuan Penelitian Dosen Muda SATEKs UnSri

Indralaya, Juli 2015

Pengaruh Fraksi Jintan

Hitam (Nigella Sativa L
.) Terhadap Proliferasi
dan Apoptosis Pada Sel
HeLa
Ziske Maritska, MD,
Msi.Med.
Sagita Darma Sari
Fakultas Kedokteran
Universitas Sriwijaya

Outline

Background
Research Questions
Methods
Results

The
Current
importance
treatment
of Cervical
of cervical
Background
Cancer
cancer

Hope for a
new cure

Options : Surgery, Radiotherapy, Chemotherapy

4th most common cancer in women world wide
(528,000 new cases in 2012)

Radiotherapy recurrence

4th most common cause of cancer death in women

(266,000 deaths in 2012 )

Chemotherapy Cisplatin (first line drug) ;

various and serious adverse effects

Kim et al., 2011

Green et al, 2015
http://globocan.iarc.fr/old/FactSheets/cancers/cervix-new.asp
http://www.iarc.fr/en/media-centre/pr/2013/pdfs/pr223_E.pdf

Herbal Medicine : where the

hope lies

Anti-tumor activity

Which
Herbs
Anti-oxidant

Less side effects

Less costly

Kalonji, Black Cumin, Habat-ul-Sauda,

Nigella
Sativa L .
Jinten Hitam
Ranunculaceae family
Self-reproducing plant with fruit capsule contaning its
seeds
Rich historical and religious background
Native to Southern Europe, North Africa, Southwest Asia;
cultivated in many countries
Extensive use of its seeds and oil for medicinal use

Gilani, Jabeen, Khan, 2004

Salem, 2005
Ahmad et al., 2013

Nigella Sativa L . ; miracles whithin

its seeds

Anti-bacterial
Various
and many active compounds has
been Anti-inflammatory
identified
Anti-viral
Anti-helminthic

Anti-oxidant

Its most important active compound and

Immunomodulator
Anti-fungal
of which
the pharmacological properties
Anti-cancer
mostly
come
from
thymoquinone
Anti-histaminic

Salem, 2005
Ahmad et al., 2013

How thymoquinone fights

cancer

El-Mahdy et al., 2005

Theoretical Scheme

Outline
Background
Research Questions
Methods
Results

The Biology
Research
of Questions
Cancer
What type of fraction of Nigella Sativa L. that has cytotoxic effect on
HeLa Cell ?
Does active fraction of Nigella Sativa L. have cytotoxic effect on HeLa
Cell ?
Does active fraction of Nigella Sativa L. have anti-proliferative effect
on HeLa Cell ?
Does active fraction of Nigella Sativa L. have apoptosis effect on
HeLa Cell ?
What active compound does fraction of Nigella Sativa L. contain ?

Outline
Background
Research Questions
Methods
Results

Methods
Will be conducted
Experimental
studyin
the
graduatecancer
laboratory
of Sriwijaya
University,
on cervical
cell line
(HeLa cell)
using
Palembang
cisplatin, ethanol extract, n-hexan fraction,
ethyl acetate fraction & ethanol-water fraction
And
from Nigella Sativa L.
Parasitology laboratory of Gadjah Mada
University, Jogjakarta

Research Workflow

Extraction, Fractination & Thin Layer

Chromatography (TLC)

Cytotoxic Test, Apoptosis test,

Cytotoxic Test
Anti-Proliferative
test

Cytotoxic test using

MTT Assay :
HeLa Cells are mixed with all
the active fractions being
studied and cisplatin
count the percentage of
cells viability

Apoptosis Test Using Flow

Cytometry

Anti-proliferative Test

Cells are incubated and monitored at 0 hour, 24

hrs, 48 hrs, and 72 hrs (doubling time) by
counting the viable cells.
After incubated results reading by using ELISA
reader

Outline
Background
Research Questions
Methods
Results

Results

Results

Results

Results

Results

Results

Financial Report
Penyusunan dan presentasi proposal penelitian
Rp. 1.500.000,
Ekstraksi dan Fraksinasi
Pembelian Simplisia Jintan Hitam 1 kg
Rp.
315.000,Pembelian Reagen
(Alkohol 96%, N-Hexan, dan Acetyl Acetat)
Rp.
380.000,Biaya Laboratorium
Biaya Penelitian dan Peminjaman Alat Gelas
Standar di Program Pasca Sarjana UNSRI
Rp.
150.000,Sewa Alat Bagian Parasitologi FK UGM
Rp.
150.000,Biaya Kultur Sel Bagian Parasitologi FK UGM
Rp.
800.000,Pembacaan Flow Cytometri
RP.
300.000,Sel HeLa
Rp. 4.000.000,Cisplatin
Rp.
500.000,Biaya Pemakaian Barang Habis Pakai (BHP)
Rp. 1.650.000,Biaya Pembelian Bahan Milik Peneliti
Rp. 1.895.000,Biaya Akomodasi
Rp. 1.692.300,Biaya Setor Pajak
Rp.
500.000,Penyusunan dan presentasi laporan kemajuan
Rp.
200.000,
Total
Rp. 14.032.300,

Thank
You

References
http://globocan.iarc.fr/old/FactSheets/cancers/cervix-new.asp
http://www.iarc.fr/en/media-centre/pr/2013/pdfs/pr223_E.pdf
Green et al. Concomitant chemotherapy and radiation therapy for cancer of the uterine cervix
(review). 2015 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. Accessed from
http://www.bibliotecacochrane.com/pdf/CD002225.pdf
Yoon et al. Treatment outcomes of extended-field radiation therapy and the effect of concurrent
chemotherapy on uterine cervical cancer with para-aortic lymph node metastasis. Clinical Radiation
Oncology. 2015;10:18
Gilani, Jabeen, Khan. A Review of Medicinal Uses and Pharmacological Activities of Nigella Sativa.
Pakistan Journal of Biological Sciences. 2004;7(4): 441-451
Salem. Immunomodulatory and Theurapetic Properties of Nigella Sativa L. Seeds. International
Immunopharmacology. 2005;5:1749-1770
Ahmad et al. A Review on Theurapetic Potential of Nigella Sativa : A miracle herb. Asian Pasific
Journal of Biomedicine. 2013;3(5):337-352
S. Zielinski. Henrietta Lacks Immortal Cells. 2010. Available at http://www.smithsonianmag.
com/science-nature/Henrietta-Lacks-Immortal-Cells.html