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Why autologous?
Autologous means persons own (self donated) and not donated
from another person or from animal origin
Growth factors must be in genetically pre-determined ratios!
No risk of rejection and lower allergenic potential
1. Formation of tri-dimensional
mesh (fibrin strand)or matrix.
3. Chemo-attraction or
migration of
macrophages and stem
cells
5. Stem cells
differentiation
Wound healing
without PRP
% Wound closure
Tissue remodeling
Tissue regeneration
Inflammation
Haemostasis
Wound healing
with
PRP
% wound closure
Tissue remodeling
Inflammation
Haemostasis
Fibrin
Fibrin
Plts
Plts agrgg
agrgg
vWF
vWF
Leukocytes
Leukocytes
Plts
Plts G.
Factors
Factors
By
By
concentratin
concentratin
gg specific
specific
cells,
cells, wound
wound
healing
healing time
time
can
can be
be
shortened
shortened
significantly
significantly
Name
PDGF aa
PDGF bb
PDGF ab
Platelet derived
growth factors
Activated thrombocytes
TGF- alpha
TGF- beta
Transforming
Growth Factors
Activated thrombocytes
IGF- I
IGF- II
Insulin-like
Growth Factors
Activated thrombocytes
EGF
Epidermal Growth
Factor
Activated thrombocytes
VEGF
Vascular Endothelial
Growth Factor
Leucosytes &
Endothelial cells
WOUND HEALING
10
% of wound closing
Healing with
PRP
Control sample
100
PAIN REDUCTION
Control sample
0
0
Journal of Oral & Maxillofacial Surgery, 2000; 58:45 Marx, Monteleone, Ghurani, Dr.
Robert Marx, University of Miami
Advantages of A-PRP
Tissue regeneration & rejuvenation: neo-collagenesis (TGF & ), neovascularisation (EGF & VEGF), & extracellular matrix formation (PDGF &
& ) NB: growth factors in genetically pre-determined ratio!
Bio-glue (fibrin glue): haemostasis & tissue adhesion in skin flaps, bone
grafts, trauma intra-surgery and post-surgery
Safety: non-allergenic & free from concerns over transmissible diseases
e.g. HIV, Hepatitis B & C, CJD, etc.
Autologous: no risk of rejection reaction
Wound healing time: increased
Physiological anti-biotic : anti-bodies & WBCs & proteolytic enzymes
Plasma includes: hormones, bio-transformed vitamins & other nutrients
Tissue engineering: in-vitro autologous tissue culture-medium.
Ease of use: dermal & hypodermal injections
Convenience: harvesting performed in doctors rooms (no external
laboratory required)
Cost effectiveness: 1 Plasma kit (2 tubes) delivers 12+ ml A-PRP
Cell separation
DENTAL MEDICINE
Dental extraction
Dental implantation
Cutaneous
reconstruction and
transplantation
Cell differential
Healing remodelling
Cardio-vascular
surgery
Abdominal surgery
Maxillo-facial surgery
Ulcer and chronic
wound therapy
(e.g. after radio
therapy)
Orthopaedic surgery
Tissue regeneration
SURGERY
DERMATOLOGY
INTERNAL
MEDICINE
GERONTOLOGY
Re-implantation of
Autologous cells,
extemporaneous or
cultivated in-vitro
Treatment of severe
burns
Skin rejuvenation:
injection (intradermis)
mesotherapy (intradermis)
topical plasma & Dermaroller micro-needling (intradermis)
2.
3.
Volumetric filling :
large volume injection of plasma intradermis and hypodermis of the tear
troughs, eyelids, naso-labial folds, marionette lines, peri-oral areas,
cheeks, forehead, glabella, neck & back of hands
A-PRP mixed with fillers such as hyaluronic acid (Esthelis, Juvederm,
Restylane, Teosyal, etc) and calciumhydroxyl-appatite (Radiesse) =
bio-active filler containing growth factors
4. Acne scarring:
subscision injections & topical plasma & skinroller/ skinneedling
5. Cellulite?
6. Striae (stretch marks)?
DERMO
COSMETICIS
PEELS
Renewal of the
corneal layer
Germinative layer
Stimulation
FILLERS
Wrinkles &
volumes
correction
BIO
STIMULATION
MyCells
Dermis Stimulation
Hyperpigmentation
removal
Architectural skin
reconstruction/
reorganization
Side-effects
Minor oedema
Seldom bruising
Eyelids can remain puffy for 2-3days
No infection
No allergy
Potential Complications
(applicable to all dermal fillers)
Contra - Indications
Why MyCells?
PRP
(Platelet Rich Plasma)
PPP
(Platelet Poor Plasma)
VEGF
220.478.1pg/
ml
72.932.5pg/m
l
EGF
PDGF-BB
269.1117.5pg 73.536.3pg/m
/ml
l
2048.4645.8p
g/ml
308125.8pg/
ml
PRP preparation(1)
PRPandPPP
Gelseparator
Mycells
tube
Centrifuge:4000 G,
G
7min.
Redblood
cell
After
centrifugation
PRP preparation(2)
PRP
Aspiration
and Blow of
PRP
Sleeve
insertion
PPP
aspiration
Forehead
Intradermal injections 0.05ml. Total
for forehead 3ml.
Upper eyelid
Subdermal injections 0.2ml each x 3.
Total 0.6ml.
Lower eyelid
Subdermal 0.2ml injections 1 cm
apart. Massage evenly. Total 1-2ml.
Cheeks
Subdermal & intradermal injections
Linear threading technique 0.2ml
per injection. Total 3-5ml per side.
Naso-labial folds
Subdermal & intradermal injections
Linear threading technique 0.2ml
per injection. Total 2-3 ml per side.
Lips
Vermillion border injections
Linear threading technique 0.2ml
per injection. Total 0.4ml per
quadrant.
Chin
Linear threading technique 0.2ml
per injection. Total 2-3ml per side.
PRP
injection
30G needle
1cc syringe
Dec. 2006
March
2007
June 2008
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