VereChip

TM

Khilda Afifah
23214316

Mengintegraskan Biochips dengan silicon heaters, temperature sensors and

miniaturized 25l volume allow the PCR module

Product

VereFlu untuk mendeteksi Influenza A: H1, H3, H5, H7, H9 with subtype IDs of H1N1, A/H1N12009, H3N2, H5N1 and H9N2 and Influenza B.

VereMTB to detect, differentiate and identify: 10 different mycobacterium strains with special
emphasis onMycobacterium tuberculosis Complex(MTBC) and its Resistance to Rifampicin
and/or Isoniazid from Pulmonary Clinical Specimens or Cultivated Samples (MDR-TB)

VereTrop to detect, differentiate and identify Multiple Tropical Diseases

VereTheat to detect, differentiate and identify Anthrax, Small Pox, Plague and Tularemia

VereFoodborne to detect, differentiate and identify multiple foodborne Pathogens in one test

Verevet to detect, differentiate and identify multiple Avian Pathogens in one test

VereMERS to detect and identifies Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Spesifikasi VereFoodborneTM

Detects multiple foodborne pathogens

Multiple Probes (with duplicates) for Bacillus spp., Campylobacter jejuni / lari /
coli, Clostridium perfringens, Cronobacter sakazakii, Listeria spp., Salmonella
spp., Escherichia coli, Shigella spp., Staphylococcus aureus, Vibrio cholera, Vibrio
parahaemolyticus, stx1A gene, stx2A gene, etc

Process controls on each chip:

PCR: Positive and Negative Controls

Hybridization: Orientation and Hybridization Probes

Limit of Detection: 100 copies of extracted genomic DNA

Sample Types**: meat, fish, vegetables, milk, water, processed foods, stool or
vomit sample

Every chip is bar-code

Integrasi VereFoodborneTM
VerePLEXTM Biosystem menggabungkan biologi
molekuler, mikrofluida dan mikroelektronika untuk
membawa masa depan diagnostik. VerePLEXTM Biosystem
termasuk komponen-komponen berikut:

Temperature Control System (5 random access

modules)

Optical Reader

Biosystem Software

Barcode Reader

Feature & Cara Kerja

Multiplex amplification
reactions

Multiple probes per target

ensures reliable detection of
subtypes in every test

Small sample volume

requirement

Fast and programmable

temperature ramp rate

PCR yield is comparable to

standard thermal cyclers

40% faster than conventional

thermal cyclers

Proprietary microfluidic
interface: contact surfaces are
biocompatible and do not
inhibit the PCR reaction

Short time required for fluidic

operations

Technology & Manufaktur

Chip silikon yang inovatif menggabungkan Micro-Electro Mechanical Systems

(MEMS) dengan mikrofluida yang memungkinkan integrasi multiplexing amplifikasi
nukleat asam dan deteksi dalam satu perangkat

The integrated silicon heaters, temperature sensors and miniaturized 25l volume
allow the PCR module to perform the rapid heating and cooling cycles required for
highly reliable, end-point PCR.

The microfluidic integration of the PCR and hybridization modules ensures that
system operation does not require highly-trained personnel, and greatly simplifies
the typical lab procedures.

Diproduksi dengan kontrol room kelas 100 dan quality control 6 sigma metodology
dan termasuk on-the fly spotting with error detection

http://vereduslabs.com/technology/technologyverechip-technology/

6 sigma metodology (DMAIC)

Defineadalah fase menentukan

masalah, menetapkan persyaratanpersyaratan pelanggan, mengetahui
CTQ (Critical to Quality)

Measureadalah fase mengukur tingkat

kecacatan pelanggan (Y)

Analyzeadalah fase menganalisis faktorfaktor penyebab masalah/cacat (X)

Improveadalah fase meningkatkan

proses (X) dan menghilangkan faktorfaktor penyebab cacat

Controladalah fase mengontrol kinerja

proses (X) dan menjamin cacat tidak
muncul

Proses Fabrikasi

A variety of structures was made using this process flow: microchannels, mixers, microreactors, micropillar filters,
capillary pumps and valves, droplet generators (for digital droplet polymerase chain reaction, or PCR), mergers,
and splitters

a) A silicon wafer (grey color) is covered with an oxide layer (green color).
b) Then, a nitride layer (blue color) is added.
c) An etch is performed to remove nitride and oxide, and silicon is excavated to form the coarse structures.
d) The exposed silicon surface is oxidized. This oxide will serve as protection during the definition of fine structures.
e) The nitride is removed and the fine structures are etched
f) the oxide is removed
g) the silicon is bonded to Pyrex
h) A backside etch is performed to open fluidic connections.

http://www.meddeviceonline.com/doc/silicon-a-material-with-huge-potential-for-lab-on-chips-0001

Contoh Teknologi dengan PCR

Pertama, sampel darah diambil
Kedua, pompa mendorong darah dan reagen ke
modul pra-amplifikasi. Modul ini terdiri dari mixer,
reaktor termal untuk PCR, dan filter.
Ketiga, dalam modul analisis, DNA dimurnikan
dicampur dengan reagen, allele-specific PCR
dilakukan sehingga hanya fragmen DNA dengan
SNP diperkuat, dan produk sampingan dari
amplifikasi terdeteksi dengan detektor
elektrokimia

http://www.meddeviceonline.com/doc/silicon-a-material-with-huge-potential-for-lab-on-chips-0001

Kelebihan

Memudahkan untuk mengontrol epidemi

pada makanan
Tes yang dipilih generik dan spesifik dari
patogen makanan yang di uji
Lab on Chip yang dirancang portable dan
dapat dioperasikan dengan mudah tanpa
membutuhkan tenaga ahli
Probe dapat di upgrade yang
memungkinkan dapat mendeteksi patogen
baru hasil dari mutasi

Perbandingan dengan Metode Konfensional

(bacteriological Analytical Manual)

Terimakasih Banyak