Beruflich Dokumente
Kultur Dokumente
(Chapter 7)
The beginning of Chapters 7 is largely a review of
topics covered in Biol 131, and our focus will be
on the sections beginning with Mutations to
Genes
Replication
Plasmids
DNA replication
Bacterial Genetics
Bacterial Genetics
Bacterial Genetics
Mutations and
evolution of bacteria
Do all mutations lead to
beneficial changes?
Do mutations create beneficial
changes in response to specific
environment stresses (such as
high temperature)?
Will a beneficial mutation
always spread through a
population?
-- under what conditions?
Bacterial Genetics
Bacterial Genetics
Three processes:
1) Transformation
2) Conjugation
3) Transduction
Bacterial Genetics
Transformation
Griffith & Avery Studies
Gene sources
Competent cells
Transformation
Bacterial Genetics
Transduction
Transfer mediated
By virus
In Specialized transduction
Specific genes are passed
Transduction
Bacterial Genetics
Conjugation
Genes carried on a F-plasmid
Process of conjugation
Loss of F+ phenotype
Plasmids can carry other genes
R-plasmids
Conjugation
Virulence plasmids
Other nonessential traits
Bacterial Genetics
10
Bacterial Genetics
11
Use of Bacteria in
Biotechnology
Role of plasmids
Cloning of useful genes
Bio-engineering of other
organisms
Genetic Engineering
Bacterial Genetics
12