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Wet Mount

MODERATOR:
Dr.Varsha A Singh
PRESENTED BY :
Dr.Khushboo gautam

Introduction

Defination:
liquid specimen/ sample suspended in saline

slide and the cover slip by surface tension.


use:
view microscopic organisms

to study their movement and morphology.

Preparation of wet mount

Specimens to be examined
Urinary tract infection-Urine
Gastro-intestinal infection-Stool, peritoneal fluid
Central nervous system infections-CSF
Respiratory tract infection-BAL, Pleural fluid
Female genital tract infection-Vaginal swab
Bone and joint infections-Synovial fluid

Urinary tract infection


Elements found in urine
Cells-RBC ,WBC, Epithelial &neoplastic cells
Infectious agents-Yeast , Bacteria
Casts
Crystals

RBC
>5RBC/HPF-hematuria
Fresh RBC-Lower UT
Haemorrhage
Inflammation
Trauma
Crenated RBC
Dehydration
Diarrhea
Heart failure
Glycosuria

WBC
>5WBC/HPF-In uncentrifuged urine
Pyuria

Squamous epithelial cells

Vaginal contamination

Transitional epithelial cells

Bladder infection

Neoplastic cells

Renal tubular epithelial cells

Renal carcinomas
Acute tubular necrosis
Heavy metal intoxication

Yeast

Bacteria

Egg of schistosoma haematobium

Cellular cast
RBC Cast

Glomerulonephritis
Renal infarction
urinary tract injury
subacute bacterial endocarditis

WBC Cast

pyelonephritis
Acute allergic interstitial nephritis

A cellular Cast
Hyaline cast

Low urine flow


Dehydration
Vigorous exercise

Granular Cast

Acute Renal failure


Acute tubular necrosis

Waxy cast

Advanced Kidney disease


Chronic renal failure

Crystals
Triple phosphate

Chronic cystitis
Chronic pyelitis

Cholesterol crystals

Nephritis
Nephrotic syndrome

Calcium oxalate dihydrate crystals

Diabetes mellitus
Chronic liver disease

Tyrosine

Severe liver disease

Drug

Sulfa drugs

Stool

Gastrointestinal tract infection

Bacterial

parasitic

Bacterial
Diarrhea

Dysentery

pus cells
Pus cells

Pus cells

RBC in rouleax pattern

Vibrio-darting motility

Shigella- dead background

parasitic
Saline
Trophozites/adult worms

Iodine
cysts & ova

Giardia lamblia
Hookworm egg

Ancylostoma duodenale

stercoralis strongyloides

Enterobius vermicularis

Ascaris lumbricoides

Charcot leyden crystals

candida

Antibiotic induced diarrhoea

Peritoneal fluid
Ascites

WBC

BRONCHO ALVEOLAR LAVAGE

pus cell
epithelial cells
Honeycomb" structures
-Pneumocystis carinii

BAL-India ink

Cryptococcus neoformins

Strept. pneumoniae

Candida albicans

Pleural fluid

Aspergillus fumigatus

Lung abscess

Tapping or draining of fluid

Trophozoite
of E.histolytica

Liver abscess
Aspirate from a space-occupying hepatic lesion

Trophozoite
Of E.histolytica

CSF

Viral infection-2-4WBC/HPF

Pyogenic bacterial infection-10WBC/HPF

Tuberculosis and mycoses->5WBC/HPF

WBC(monocyte)

Lymphocytes under methylene blue


preparation

candida

India ink preparation

C.neoformins

Strept. pneumoniae

Naegleria fowleri

Acanthamoeba

vaginal swab

Motile trophozoites of trichomonas vaginalis


saline preparation

Vulvovaginal candidiasis

pseudo hyphae or budding yeast -saline


wet preparation

Bacterial vaginosis

Presence of >20% per HPF of clue cells on


wet mount examination

synovial fluid

Non inflammatory

-1 to 2 WBC/HPF

Inflammation or infection

->20 WBC/HPF

Septic
- >100WBC/HPF
(also Rheumatoid arthritis,gout,pseudogout)