Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and

Sciences

Blood Collection
Reference: HENRY, Harmening

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Topic Outline
I. Donor Registration
II. Donor Selection
III. Medical History
IV. Basic Qualifications of the Potential Blood Donor
V. Deferral
VI. Phlebotomy
VII.Donor Reactions

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Donor Registration
First step
Consent
Personal Information

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Donor Selection
Two Broad Questions:
1. Might the procedure be harmful to donor?
2. Might the donors blood be a risk to the recipient?

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Medical History and

Miniphysical

Medical Questionnaire
Physical Examination

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Basic Qualifications of the

Potential Blood Donor
General Appearance
Age: 18-59 years old (60-65, for regular donor)
16-17 years old must have parents consent
Temperature: 37oC

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Basic Qualifications of the

Potential Blood Donor
Body Weight: 110 lb (50kg) for 450mL collection
How to compute for 10.5mL basis collection?
>

Amount of Blood to be drawn:

Donors weight (lb) x 450 mL

= Allowable amount (mL)
110 lb
> Amount of Anticoagulant needed:
Allowable amountx 14
= Anticoagulant needed (mL)
100
> Amount of anticoagulant to remove:
63 mL anticoagulant (mL)
= Anticoagulant to remove
(mL)

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Basic Qualifications of the

Potential Blood Donor
Pulse: 50 to 100 bpm
Blood pressure : between 90-140mmHg (systolic)
between 60-100 mmHg (diastolic)
Hemoglobin: 125g/L (12.5g dL)
Allogeneic Donation
greater or equal to 12.5 g/dL (hemoglobin)
greater or equal to 38% (hematocrit
Autologous Donation
H/H: greater than or equal 11 g/dL and 33%

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Permanent)
Men who have had sex with another man anytime
since 1977
Hemophiliacs
Intravenous drug abusers (past or present)
Persons who have engaged in sex for money or drugs
any time since 1977
Positive for AIDS
Symtoms of AIDS
History of viral hepatitis after age 11

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Permanent)
Confirmed positive test for hepatitis B surface antigen
(HBsAg)
Positive for hepatitis B core antibody
Confirmed positive test for hepatitis C antibody
Confirmed positive test for Human T-cell lymphotropic
virus (HTLV)
Malignant solid tumors (except for basal cell carcinoma of
the skin and carcinoma in sit of the cervix.)

Hematologic malignancies
Chronic cardiopulmonary, liver, or renal disease

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Permanent)
Those who have ever taken the drug etretinate
(Tegison) for the treatment of psoriasis.
Donors who received pituitary-derived human growth
hormone
Person who received a tissue transplant of cornea or
dura mater

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Temporary)
Active disease under treatment such as cold, flu,
tuberculosis, syphilis, infections;
curable diseases of the heart, lung, kidney, liver, and
gastrointestinal tract;
and treatment with antibiotics

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Three Years)
An immigrant or refugee coming from an area
considered endemic for malaria, three years after
departure,
or those who have had diagnosis of malaria, three
years after becoming asymptomatic

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(One Year)
After hepatitis B immune globulin administration,
after therapeutic rabies vaccination (after bite from an
animal)
Rape victims
Healthcare workers
Percutaneous exposure to blood or body fluids,
Close contact with viral hepatitis
Tattoo,
Sexual contact with a prostitute or other persons in a

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(One Year)
Incarceration in a jail for more than 72 consecutive
hours
Transfusion of blood components
Travel to endemic for malaria (with or without
prophylactic therapy)

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
( Two Months)
Recent blood donation

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Six Weeks)
Following delivery of a baby

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(One Month)
German measles (rubella) vaccination
After cessation of the drug isotretinoin (Accutane) for
acne treatment
After cessation of the drug finasteride (Proscar) for
treatment of benign prostatic hyperplasia

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(Two Weeks)
After immune reaction to smallpox vaccination
After vaccination with

oral polio,
measles (rubeola),
Mumps or yellow fever

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Deferral
(48 hours)
Whole blood donation deferred after hemapheresis

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Donor Reaction
Mild Reactions

Syncope or fainting, nausea, or vomiting, hyperventilation,

twitching and muscle spasm.

Moderate Reactions

Loss of consciousness in addition to mild reactions.

Severe Reactions

Presence of convulsions

Cerebral ischemia
Marked hyperventilation
Epilepsy

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences

Immunohematology (Blood Banking)

Department of Medical Technology, Institute of Arts and
Sciences