Beruflich Dokumente
Kultur Dokumente
NA Denaturation
Outlines :A.
B.
C.
D.
E.
F.
G.
Introduction.
What's meant by DNA Denaturation.
Causes of DNA Denaturation.
Characters of Denatured DNA .
Diseases caused by DNA Denaturation.
DNA renaturation.
Application on DNA denaturation and
renaturation.
H. References.
I. Participants.
A. Introduction
-DNA is a double
helix composed of 2
strands running in
opposite directions .
-Each strand composed
of backbone of deoxyribose sugar
molecules linked
together by phosphate
linkage .
or template for
particular sequencing of amino acid that
bond together and make protein.
B.DNA Denaturation
Definition
C.Causes of
Denaturation.
Denaturation can occur when
proteins and nucleic acids are
subjected to :
1-elevated temperature.
2-extremes of pH.
3-nonphysiological
concentrations of salt, organic
solvents, urea, or other
chemical agents.
.1-elevated temperature.
The most common
type of denaturation
is thermal
denatuation.
Tm : temp at which
DNA is half
denatured.
*above Tm DNA is
single strands.
*below tm DNA is
double strands.
C.2-extremes of pH
At high PH the
hydroxide
ions (( negatively
charged
ions )) can pull hydrogen
ions from base pairs
forming H bond between
two strands causing
them to separate.
D.
F.DNA Renaturation
Renaturation or annealing :
I.
G.References
1.
2.
3.
4.