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Photosynthesis
-converts sunlight into chemical energy
-very complex
-general reaction:
6CO2 + 6H20 C6H12O6 + 6O2
380 nm
750 nm
PHOTONS
-Photon=quantum=discreet amounts of light
energy
-Photons are not objects, but each one has a
distinct amount of energy related to
wavelength frequency
Ex: violet photons contain almost twice as
much energy as red photons
*violet wavelengths=380 nm=high
frequency=high energy
*red wavelenghts=750 nm=low
frequency=low energy
Absorption Spectrums
Absorption spectrums are graphs that plot a pigments light
absorption vs. wavelength
Absorption spectrum of chlorophyll
**Remember:
Green wavelengths
are between ~475
and 600 nm
Action spectrums
A. Action spectrums tell you how much photosynthesis is
occurring at each wavelength
B. Made by illuminating chloroplast with different
wavelengths of light and then plotting wavelength against
some measure of photosynthetic rate
C. The photosynthetic rates could be measured by finding
oxygen production, carbon dioxide absorption or light
Action spectrum for chlorophyll
absorption
Action spectrum
for chlorophyll
*Almost no absorption at
green wavelengths
ASSIGNMENT
1. READ: Photoexcitation of chlorophyll
(p. 175)
2. Look at the pics and read the annotations
for additional explanations
3. Briefly outline the section
4. Draw/label/annotate a chloroplast as seen
in an electron micrograph.
C. Oxygen production
1. Photosystem II absorbs light
2. Its electrons become excited
3. Photosystem II donates its electrons to an electron
transport chain and the flow of electrons will generate
an ATP molecule
4. Photosystem II has been oxidized (LEO)
5. To get the electrons back (that were donated) an
enzyme in the center of photosystem II breaks a
water molecule
D. ATP Production
1. an excited electron from the center of PS
II is donated and passed along a chain of
electron carriers
2. The energy for ATP is generated via a
proton gradient that is created as electrons
move through an ETC (chemiosmosis)
E. NADPH Production
1. NADPH = nicotinamide adenine
dinucleotide phosphate-oxidase
2. After PS I accepts the electrons that were
donated by PS II (the ones that went through
the ETC), PS I becomes photoactivated
3. Next PS I donates its excited electrons to
NADP+ reductase via another ETC
4. NADP+ reductase is an enzyme that
assists in the reduction of NADP+
NADP+ reductase
NADPH
F. Cyclic photophosphorylation
(in the book it is cyclic electron flow)
1. PS II is not involved
2. Produces ATP but not NADPH
3. ATP is made via chemiosmosis (the same way
as non-cyclic photophosphorylation)
4. How it works:
a. PS I absorbs light
b. the excited electrons are given to an electron
acceptor
c. the electrons pass through an ETC to
produce ATP via chemiosmosis
d. At the end of the ETC the electrons go back
to PS I and the process starts again
Assignment
#1. Draw and annotate a chloroplast.
-Include: grana
thylakoids
thylakoid membrane
stroma
ribosomes
double membrane
circular DNA
fat/oil droplets
HOMEWORK
Complete the coloring worksheet on
light reactions
TURN IT IN TODAY (2-19)
GET YOUR BOOK
GO TO P. 180-181
Light-independent reactions
(light not required)
Rubisco
3CO2
6 glycerate-3-phosphate
**For every RuBP and CO2, two three carbon molecules are formed
B. Reduction
1. molecules of glycerate-3-phosphate are
phosphorylated to glycerate-1,3-biphosphate
*when ATP is hydrolyzed to ADP
2. glycerate-1,3-biphosphate is reduced when
NADPH donates its electrons
*NADPHNADP+
3. 6 molecules of triose-phosphate are produced
*one is removed from the Calvin cycle and
used by the plant to produce sugar/organics
*the other 5 are recycled back into the Calvin
cycle and converted back to 3RuBP
Measuring Photosynthesis
*can be done three ways
A. Production of oxygen
1. Aquatic plants release oxygen in
bubbles during photosynthesis
2. If the bubbles are collected, their
volume can be measured
C. Increase in Biomass
(measure the increase in sugar molecules)
Light intensity
Rate of photosynthesis
Rate of photosynthesis
Temperature
Review Questions
1. Compare action spectra and absorption spectra.
2. What are the functions of ATP and NADPH
produced in non-cyclic photophosphorylation?
3. What is the purpose of cyclic
photophosphorylation?
4. What is the advantage of non-cyclic
photophosphorylation over cyclic
photophosphorylation?
5. What is the purpose of the Calvin cycle?
6. Explain the relationship between the structure of
the chloroplast and its function.