Thermodynamic stability:

temperature;
pH;
composition.
Lipid composition;
Protein composition;
Diffusion capacity;
Protein channel;
Vesicle labeled;
receptor;
lectin;
antigen;
immunoglobulin;

Hidrofob

Hidrofil

Amfifil

Spaiu hidrofob

Micela - monostrat lipidic

Mediu apos

Vesicle Liposome: amphiphilic compounds nature

Concentric bilayered
Hydrophilic - entrapped
Hydrophobic - diffusion

Hydrophilic area

Vesicle

Water environment

Protein channel
Ions

Binding capacity
Cell: imitation, adhere

Low molecules
S; P

Drug delivery
Labeled capacity
receptor;
lectin;
antigen;
immunoglobulin;

Hydrophilic area
(Enzyme)

Vesicle

Water environment

Vesicle possible applications

Treatment of myocardial infarction:
SBP / fibrin clots: receptor interaction;
Vesicle degradation
free PActivator
PA
Plasminogen
Plasmin
Plasmin
Fibrin clots
monomer fibrin
PActivators:
- Streptokinase;
- Urokinase;
- Tissue plasminogen activator.

Fibrin clots degradation

Surface bond
Plasminogen
(SBP)
(PA)

Monomer fibrin
Fibrin clots Fibrin clots
plasmin

Plasminogen receptor
Vesicle instability

degradation

Treatment of diabet
Glucose permeation;
pH vesicle instability;
Insulin vesicle permeation ;
Dynamic glucose determination

Blood

Blood

Insulin +GOs
Glucose

Acid G

pH

Insulin +GOs
Insulin
Glucose cell permeation
for oxidation

Analytical determination of microbial infections

Ag-Ac interactions;
Complex Ag-Ac
Vesicle instability;
p-NO2P; p-NO2OH - Vesicle impermeability
PAL outside of vesicle
Spectrophotometric determination (p-NO2OH)
Intensity of coloration = f(Specific interaction
number)

Antigen
Antibody

PAL

p-NO2P

PAL

p-NO2P

PAL
p-NO2OH

Spetrophotometric
determination

Px
Aromatic
amine

Px

Aromatic
amine

Px

p-quinone

Spetrophotometric
determination

Cation decontamination of industrial water

Cd2+, UO22+
Analytical control with atomic absorbtion
In situ Pi synthesis: glycerol-P vesicle permeation
pH modification: Me-precipitation
inside (Me channel)
outside (Phosphate channel)
Vesicle permeation: Me
Pi
Vesicle stabilization

Phosphatase
Phosphatase

Glycerol-P

Mn+

Phosphatase

Pi

Mn+

Insoluble complex

Antigen-antibody interaction determination

Immunoglobulin vesicle labeled;
Antigen coating on inert surface (well)
ELISA application

E
S
P

In vitro glucose determination

vesicle adsorbtion on inert surface (well);
vesicle permeation: glucose, S, P
P light absorbtion modification

GO, Px

Glucose

GO, Px
H2O2

GO, Px
P

Redox potential and ROS degradation

Xantine or hypoxantine determination

XO, SOD
Px
H2O2

XO, SOD
Px
X

Inflamation limitation:
Adding Px and SOD in tissues

XO, SOD
Px
HOH

Redox potent

Xenobiotic degradation in vivo/in vitro:

Permeability of vesicle: NADP+; X; XP

Cyt P450
G6PDH
NADP+ X
G6P

Cyt P450
G6PDH
NADPH
X

Cyt P450
G6PDH
NADPH
Xox

Cyt P450
G6PDH
NADP+
X

Fluorescence

G6PDH: NADPH regenerated

UV-VIS absorbtion

Cyt P450 monooxigenase activity

ELISA

Vesicle surface (membrane) immobilized

Glucose: possible sensor

H2O2

GO

Glucose

Px