Beruflich Dokumente
Kultur Dokumente
of E. coli
Nazish Baig
BSBT - 02113028
CONTENTS
What is E. coli?
Types of E. coli
Virulence of E. coli
Epidemiology
Virulence genotype
Adhesins Virulence genes
Serum Resistance protein
Siderophores
Toxin and Proteases
Summary
Enterobacteriaceae family
Gram Negative and rod shaped and
normal flora
Remain unaffected
No virulent gene
Become virulent and cause disease
Bacteriophage
Plasmid DNA encode
enterotoxins
Virulence factors invasion
TYPES OF E. coli
Six pathotypes
1.
2.
3.
4.
5.
6.
DAEC
EPEC
EHEC
EAEC
ETEC
EIEC
EPIDIMOLOGY
VIRULENCE
FACTOR??
VIRULENCE FACTOR
Promotes
Proliferation
Colonization
Interaction
Type 1
fimbriae
Surface virulence
factors
LPS
P fimbrial
(lipopolysacch
aride)
S and F1C
fimbriae
Haemolysin
(HlyA)
cytotoxic
necrotizing
factor
(CNF)
siderphore
s
iron
molecules
Genotype
SURFACE VIRULENCE
Adhesins
Adherence of bacterium with host
Type 1
fimbriae
P fimbriae
S fimbriae
Afimbrial
adhesins
FimH, FimA
D mannose sensitive
type 1 fimbriae to join or attach with various cells,
such as, epithelial cells, erythrocytes, mast cells,
granulocytes macrophages
sfa/foc
Central region of sfa (S fimbriae) and foc (F1C
fimbriae) operons
Afimbriated
Bacterial Cells
Non-adherent
Afimbriated
Bacterial Cells and
Buccal Cells
Fimbriated
Bacterial Cells
Iss gene
EXPORTED
VIRULENCE
Siderophores
Conti..
Haemolysin (hly)
Hemolysin genes
Hly
A
Hly
B
Hly
C
Hly
D
programs the
structure of
haemolysin
protein
gives energy
during the
process
activates hlyA,
so enable
binding with
erythrocytes
release of
haemolysin
from the outer
membrane
Hemolysin genes
Surface Coating
LPS
(lipopolysacch
aride)
O
antigens
K antigens
(capsular
polysaccharides)
Summary
E. coli is the most common and prevailing gram
negative bacteria mostly found in human fecal. Some
theories on pathogenicity suggests that distinct
properties which enables the E. coli to overwhelmed
the host defense in new environment that is needed
to escape the restriction of the colonic mileu, moves
in the new niches
Virulence factors help to discriminate or characterize
the potential of pathogens from normal strains
In this presentation we summarized the current
position of knowledge about the genetics,
mechanisms of action, and clinical significance of the
VFs thought to be most important in E. coli.