Beruflich Dokumente
Kultur Dokumente
X 1000
(mm)
(m)
(nm)
2mm
130m
0.13
0.032m
32
7.25m
0.00725
(2 x 106)
130
130000
(1.3 x 105)
32000
32000000
(3.2 x 104)
(3.2 x 107)
7.25
7250 (7.25 x
103)
1000
1000
Learning Objectives
- [PA] use an eyepiece graticule and stage
micrometer scale to measure cells and be
familiar with units (millimetre, micrometre,
nanometre) used in cell studies;
-[PA] calculate linear magnification of
drawings and photographs;
-(h) [PA] calculate actual sizes of specimens
from drawings and photographs;
Measuring cells
The graticule
a more suitable ruler for measuring cells
stage graticule
e.g. x100
stage graticule
e.g. x400
stage graticule
Low magnification
High magnification
Figure 4.3
Stage micrometer viewed at x100 magnification.
The total length of the micrometer is 1mm
on this scale, 94
divisions = 1000m
Figure 4.1
Cells of onion epidermis as viewed at x100
magnification with a graticule in the eyepiece of the
microscope
We
know
at thiscovered
In the
twothat
columns
magnification,
division
by the graticule1 there
is an
on
the eyepiece
graticule
average
of five cells
in the
represents
10.6
m
length of the
graticule
1060m
Therefore
the
average
Therefore
the
total
length
of one cell
is
oflength
the eyepiece
graticule
1060 5 =10.6
212m
represents
x 100 = 1060m
at this magnification
Figure 4.4
Part of the stage micrometer viewed at x400
magnification
remember
thatshown
each
so the length
byhere
the bracket
is
division
is 10m
240m
on this scale, 90
divisions = 240m
Figure 4.2
Cells of onion epidermis as viewed at x400
magnification with the same graticule in the eyepiece
Figure 4.6.
Cells of the onion epidermis as viewed at x400
magnification with the same graticule in the eyepiece
of the microscope
62 divisions