Beruflich Dokumente
Kultur Dokumente
Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115 USA
2
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland
ABSTRACT
o This study seeks to identify whether Broad
bean mottle virus (BBMV) and Cowpea
chlorotic mottle virus (CCMV), members of
family Bromoviridae, misencapsidate host RNA
segments.
o RNA viruses like Flock house virus (FHV) are
known to encapsidate host RNA by mistake.
o Preliminary data suggests that Brome mosaic
virus
(BMV),
a
bromovirus,
might
misencapsidate host RNA as well.
o Broad bean (Vicia faba) and cowpea (Vigna
sinensis), natural hosts for BBMV and CCMV,
respectively, were used to cultivate the
viruses.
o Virion extraction and purification remove
cellular RNA and DNA fragments. This is
followed by the extraction of viral RNA.
o Finally, the viral RNA extracts are sequenced
by Next Generation RNA sequencing (NGS-RNA
Seq) and then are BLASTed for final analysis.
o The result should contribute to a better
understanding of the virus-host relationship
and the role of viruses in horizontal gene
transfer (HGT).
BACKGROUND
BACKGROUND (cont.)
DISCUSSION
1.
B
1.
A
The genomes of BBMV and CCMV. 1.A) The genome of BBMV. 1.B) The genome of CCMV
BBMV
inoculated to
broad beans
(Vicia faba)
CCMV
revitalized
in
Chenopodium
quinoa
CCMV
inoculated to
cowpeas
(Vigna
sinensis)
Extraction and
vigorous
purification
Extraction and
vigorous
purification
RESULTS
2.
B
2.
A
NextGen
Sequencing at
the University
of Chicago;
Sequence
analysis
NextGen
Sequencing at
the University
of Chicago;
Sequence
analysis
2.
C
2.
D
Preliminary RNA Gel of BMV and Infection of Host Plants. 2.A) This gel of the BMV genome shows
three bands of the actual viral genome as well as a faint band that could be misencapsidated host RNA.
2.B) Local lesions in C. quinoa caused by BBMV. Three of these lesions have been emphasized by black
circles 2.C) This figure depicts the systemic infection of BBMV in broad beans. Symptoms of infection
include the large yellow-green patches that follow venation of leaves (Photo source: Freeman, 2006). 2.D)
The symptoms of a systemic CCMV infection in a cowpea leaf can be seen. Symptoms are characterized by
yellow-green spots and lines spread across the leaves of the entire plant.
ACKNOWLEDGEME
NTS
CITATIONS