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Antibiotic Resistance

The burden of antibiotic resistance bacteria has


been increasing over the years and has been a
major health problem in the last decades

The deleterious effect of multidrug resistant


bacterial infection has been increasing the level of
mortality

This resistance has impacted on the excess


number of deaths, bed-days, and hospital costs

The need to find a cure has never became so


apparent

Antimicrobial Activity and


Synergistic Antibacterial Effect of
Curcumin Re-sensitize Resistant
Strain of Bacteria

Mohammad Vico Rizkita Wisnu Laksana


Rochelle Vernique Siem

Introduction
Materials and Method
Results and Discussion
Conclusion

Curcumin
Curcumin (diferuloylmethane) is an
active ingredient of turmeric isolated
from the roots of Curcuma longa.
It is commonly used as spices and
traditional drink (Jamu)

What is the
objective?

MRS
A

P.
aeruginosa
ESBL
positive K.
pneumoniae
& E. coli
MDR A.
baumannii

High dose of
curcumin up to 8
grams/day DO
NOT exhibit any
toxic effects in
human health

Introduction
Materials and Method
Results and Discussion
Conclusion

Initial citation
lists from all
databases by
key words
(n=1610)
n=1426 excluded: not
an original research
article, not fitting key
terms of inclusion
criteria

Google
Scholar
Initial
screening
of
citations
n=171 excluded: title
(n=184)
not fitting key terms
of inclusion criteria,
only explained
curcumins effect as
general

Scienc
e
Direct

Secondary
screening of
citations
from
n=3 excluded: do not
abstracts
(n=13)provide enough data,
using ultrasound and
photodynamic therapy,
and requires purchase

Full text
journals
that
were
analyzed

Introduction
Materials and Method
Results and Discussion
Conclusion

Synergistic antibacterial effect


of curcumin against MRSA

Figure 1 Time-kill curves of


Staphylococcus aureus using curcumin

Inhibition of bacterial growth of MRSA by curcumin encapsulated


nanoparticles in infected burn wound murine model

Figure 2 curcumin nanoparticle decrease


bacterial burden of full-thickness burn on
day 3 (A) and day 7 (B) in murine model
infected with MRSA

Secretion of PBP2a Protein after


combination of Curcumin and
Oxacillin
Figure 3 Western blot analysis of
penicillin binding protein (PBP)2a
production by ATCC 33591 after
treatment with 32 g/mL oxacillin
(OX) and 250 g/mL curcumin
(CCM). The control (CON) was used
without treatment drugs

Effect of Curcumin against Quorum sensing and


Biofilm formation of Pseudomonas aeruginosa

Figure 4 Results of
curcumin in levels of
Catalase (A and C),
SOD (B and D), and
sensitivity to H202 (E)

Combination of Curcumin and Imipenem against ESBL


Positive E. coli and K. pneumoniae
Code of
bacteri
a
E6(+)
E34(+)
K1(+)
K4(+)
K5(+)
K6(+)
K7(+)
K8(+)
K2(-)
E25(-)
E41(-)

Imipenem
MIC
(g/mL)

Imp/Curcu
min MIC
(g/mL)

4
64

1
1

Decrease in
MIC
(number of
dilution)
2
7

8
8
8
4
8
16
4
4
4

0,5
1
1
1
1
1
2
0,5
1

4
3
3
2
3
4
1
3
2

Table 1 Imipenem and


Imipenem/Curcumin MIC
values. E: E. coli, K: K.
pneumoniae, (+): ESBL
positive, (-): ESBL
negative

Combination of EGCG and Curcumin against MDR


Acinetobacter baumannii
ATCC 19606

AB292

Figure 5 Time-kill curve of Acinetobacter


baumannii strain ATCC 19606 (above) and
AB292 (below) versus of CCM, EGCG and
combinations of both compounds

Introduction
Materials and Method
Results and Discussion
Conclusion

Curcumin, a natural polyphenolic compound possess a


potent antibacterial activity against resistant strain of
bacteria in various species
The combination of curcumin and antibiotics could be
developed as a new and safe strategy against multidrug
resistant bacteria.
Further in vivo research regarding the bioavailability
and pharmacodynamics activity of curcumin is
compulsory for developing effective treatment in the
future

Thank You!

References
1.

Lakshmikantha M, Asthanaashish, Molly M. Antimicrobial Activity of Curcuma Longa Extracts on MethicillinResistant Staphylococcus aureus (MRSA). Journal of Advance Researches in Biological Sciences, 2014, Vol. 6
(3&4) 213-216.

2.

Mun SH, Joung DK, Kim YS, Kang OH, Kim SB, Seo YS, et al. Synergistic antibacterial effect of curcumin
against methicillin-resistant Staphylococcus aureus. J Phymed. 2013; 20(89): 714718.

3.

Mun SH, Kim SB, Kong R, et al. Curcumin Reverse Methicillin Resistance in Staphylococcus aureus. Molecules.
2014, 19, 18283-18295; doi:10.3390/molecules191118283.

4.

Sethupathy S, Prasath KG, Ananthi S, Mahalingam S, Balan SY, Pandian SK. Proteomic analysis reveals
modulation of iron homeostasis and oxidative stress response in Pseudomonas aeruginosa PAO1 by curcumin
inhibiting quorum sensing regulated virulence factors and biofilm production. Journal of Proteomics.
Forthcoming 2016. doi: 10.1016/j.jprot.2016.04.019

5.

Gunes H, Dogan M, Gumus A, Mutlu R, Topkaya AR. Investigation of synergistic effect of curcumin with some
antibiotics in extended spectrum beta lactamase positive or negative Escherichia coli and Klebsiella
pneumoniae isolates. Acta Mediterania. 2013, 29: 761.

6.

Betts JW, Wareham DW. In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG)
versus multidrug-resistant Acinetobacter baumannii. BMC Microbiology. 2014, 14:172.

7.

Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal Activity of Curcumin I Is Associated with
Damaging of Bacterial Membrane. PLoS ONE. 2015. 10(3): e0121313. doi:10.1371/journal.pone.0121313.

8.

Gunes H, Gulen D, Gumus A, Mutlu R, Tas T, Topkaya AR. Antibacterial effects of curcumin: an in vitro
minimum inhibitory concentration study. Toxicol Ind Health. 2013. DOI: 10.1177/0748233713498458.