Beruflich Dokumente
Kultur Dokumente
- Overview of bioreactors
- Modified batch and continuous reactors
- Scale up/down
- Immobilized cell systems
- Operation consideration
- Sterilization
- Bioreactor Instrumentation and control
- Classification:
operation modes:
- batch: stirred tank
- continuous: chemostat, fluidized-bed
- modified types of the above modes: fed-batch,
chemostat with recycle,
multi-stage continuous reactors
Oxygen supply:
- aerobic: airlift
- anaerobic
Form of biocatalyst:
- free cell (enzyme)
- immobilized cell (enzyme)
packed-bed, membrane reactor
Industrial Bioreactor
X1, S
where =net=g-kd
When kd=0
FS 0 FS V g X 1
dS
(1 ) FS V
dt
YX / S
X1
Y X / S (S0 S )
1 C
, X1
YX / S
[1 (1 C )]
[S0
K s D(1 C )
]
m D(1 C )
No recycle
X1, S
(1 ) FX1 FX 2 FCX1 X 2
The average residence time in cell separator
Vcell separator
(1 ) F
Fed-Batch
Nutrients are continuously or semi-continuously fed,
while effluent is removed discontinuously.
- overcome substrate inhibition or catabolite repression by
intermittent feeding of substrate by maintaining low substrate
concentration.
for production of secondary metabolites e.g. antibiotics, lactic
acid, E. Coli making proteins from recombinant DNA
technology.
Fed-Batch
Analysis of fed-batch with substrate continuously fed
and no output: t=0, V0=0, F is constant.
dV
F V Vo Ft
dt
At quasi steady state, S added=S consumed,
X, S, P concentrations are constant.
Volume:
d ( XV )
dX
dV
V
X
, then
dt
dt
dt
FX O V net X V
V net X X
dX
dV
X
dt
dt
dV
1 dV F
net
D
dt
V dt V
F
F
net
V
net D
m S
if K d 0
Ks S
Do
1 D0 t
Then S
Ks D
m D
V0 Ft
Fed-Batch
where S0
assuming XXm
M
Fed-Batch
(g)
qp (i.e. g product/g cells-min)
at Pt=Pt0, t=0
Fed-Batch