STANDARDIZATION OF ETHYL ACETATE

EXTRACT OF ANTING-ANTING (ACALYPHA

INDICA L.) AS ANTIMALARIAL HERB
By: Hilmatul Rosyidah (12630032)

Supervisor : Elok Kamilah Hayati, M.Si

Consultant: Roihatul Mutiah, M.Kes, Apt
Religion Supervisor : Nur Aini, M.Si

Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

Background of the research

Dewoto, 2007

Qs. Asy Syuara (26) :7

(the useful of plants)

The secondary
metabolit content

Husna, 2011

The Efficiency Ethy acetate

extract of Acalypha indica as
antimalarial = 87,7% (in
vivo test)
ED50 = 3,083 x 10-26
mg/Kg

Musthofa, 2009

Acalypha indica L. can

be develope as an
officially standarized
herbs, especially as
antimalarial through
the standardization
proses.

Specific parameters:
determine of the
compound/ component
that has specific funciont
to certain pharmacological
activity
Non-specific parameter:
determain the external
factor that can affect the
stability & safety of
consumers.

Heinrich, 2005

The usefulnes of
medicinal herbs in
Indonesia hasnt been
done officially

INTRODUCTION
Problem of Reseach

Problem 1st

Problem 2nd

How the result of specific parameter test from Acalypha

indica L. extract?

How the result of specific parameter test from

Acalypha indica L. extract ?

Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

INTRODUCTION
Foccus

The sampel used was fresh Acalypha indica L. from DinoyoMalang.

The solvent used in maceration process was ethy acetate (p.a).
The specific parameter tests are; phytocemical test of akaloid
compund by using Dragendorff and Meyer reagent, determination
of total alkaloids, marker compound (berberin), content of water &
ethanol soluble extract.
Non-specific parameter tests are ; the estabilishment of specific
dencity of the extract, moisture content and ash residual solvent,
and determination of heavy metal contamination of lead (Pb).
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

INTRODUCTION

Advantage of research

The result of this research are

expected to provide an overview of the
characteristics and standardization of
Anting-anting extract can be used as
an officially standarized herbal
medicine, and also can be widely used
by Indonesian people.
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

THEORY
The Classification of Acalypha indica Linn.
(Hutapea, 1993; BPOM RI, 2010)

Kingdom : Plantae
Division
: Spermatophyta
Sub Division
: Angiospermae
Class : Dicotyledoneae
Ordo
: Euphorbiales
Family : Euphorbiaceae
Genus : Acalypha
Species : Acalypha indica Linn.
Trade name : Kucing-kucingan
Synonyms : A. spicata L., A. Ciliate L., A. canescana L., A. australis
L., A. canescens Wall.

THEORY
Standardization..
SPECIFICT PARAMETER
TEST
Non-SPECIFICT
PARAMETER TEST
Standardization: the process of development and agreement of
technical standards through a rigorous experiments and
observations that produces a set of characteristics herbal
remedies that are safe for consumption with a good quality
(Kunie et al., 2012).

STEPS OF
RESEARCH
sampling

Sample
Preparation

Analysis of
Data

Non-specific
parameter
test

Preparation of
extract
Specific
parameters test
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

RESULT AND DISCUSSION

The Making of Ethy Acetate Extract of Acalypha indica L.

Rendement: 4,51%
(w/w)

Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

The Result of
Specific Parameters Test
No.

Parameter

Result

1.

Water soluble compound

9,548%

2.

Ethanol soluble compound

79,612%

3.

Alkaloid test by using

a. Dragendorff reagent
b. Meyer reagent

4.

Total alkaloid test

5.

Berberine marker compund

(+)
(+)
68,2577%
33,59%

The Result of
non-Specific Parameters Test
No.

Parameter

Result

Rule

17,9497

5 30 %
(Voight, 1994)

1.

Water content

2.

Ash content

1,978

3.

Solvent (ethy acetate)

0,998

1,0
(BPOM RI, 2006)

4.

Lead (Pb) content

4,46 g/Kg

10 mg/Kg
(BPOM, 2006)

Study of Islamic
Qs. Yasin (36): 35. 1

?That they may eat His fruit, and their hands have produced it, so will they not be greatful
2. Ali Imran (3) : 190 191:

Indeed, in thecreation of the heavens and the earth and the alternation of the night and the day are
signs for those of understanding. Who remember Allah while standing oe sitting or (lying) on their
side and give thought to the creation of the heavens and earth, (saying), Our Lord, You did no
create this aimlessly; exalted are You (above such a thing); then protect us from the punishment of
the Fire

Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

Study of Islamic
3. Qs. Al Furqan (25): 2



He to whom belongs to the dominion of the heavens and the earth and who
has not taken a son and has not had a partner in dominion and has created each
thing and determined it with (precise) determination.

CONCLUSION
The results from the specific parameters
test of the ethyl acetate extract of
(Acalypha indica Linn.):
content of water soluble compounds
as much as 9.548% 0.527,
ethanol-soluble extract 79.62167%
1.902.
Alkaloid content with reagents
Dragendorff and Meyer showed
positive results.
Levels of total alkaloids in the extract
obtained by 68.2577% 3.648.
Compound berberine marker
positively identified at tR 15.76 and
19, 016 minutes by levels of as much
as 33.59%.
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang

The test results of non-specific

parameter:
water content of as much as 17,9497
0.6656%,
ash content as much as 1.978%
0.3153,
the levels of residual solvents (ethyl
acetate) and 0.9989 0.00782
Pb metal contamination levels of
4.46 g/Kg.

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COMPARISON BETWEEN HPLC & UPLC

Parameters

HPLC Assay

UPLC Assay

Column

XTerra,C18,50 4.6mm

AQUITY UPLC BEH C18,50 2.1mm

Particle size

4m particles

1.7m particles

Flow rate

3.0 ml per min

0.6 ml per min

Injection volume

20 l

3 l partial loop fill or

5 l full loop fill

Total run time

10 min

1.5 min

Theoretical Plate count

2000

7500

Lower limit of quantization

0.2 g/ml

0.054l/ml

Total solvent consumption

Acetonitrile:10.5ml,
water:21ml

Acetonitrile:0.53ml,
water:0.66ml

Delay volume

720 l

110 l

Column temperature

Resolution

30 C
35-40 Mpa
less
Less

65 C
103.5 Mpa
more
High

Method development cost

High

Low

Maximum back pressure