SINGLE WALLED CARBON NANOTUBE BASED CHEMIRESITIVE/FET

BIOSENSOR FOR THE DETECTION OF BIOMARKERS

Submitted by
(Binay Kumar Prasad)
Nuclear Science and Engineering (NSE)
(2K14/NSE/17)

Department of Applied Physics

Delhi Technological University
(Formerly Delhi college of Engineering, DCE)
New Delhi-110042 (2014-2016)

CONTENTS
Biosensor
Types of biosensor
Steps/Process
Materials/Apparatus
Results
Conclusion

Biosensor
ANALYTE

antibod
y
BIORECEPTOR

MOLECULAR
RECOGNITION

FET act as a transducer in

biosensor

TRANSDUCER

MEASUREMENT

Types of Biosensor (FET based)

MOSFET based biosensor
ISFET
Carbon Nanomaterial FET
Carbon nanotube FET
Graphene FET

FET as biosensor

Process involved
DIELECTROPHORETIC ALIGNMENT OF SWNT
BETWEEN SOURCE AND DRAIN

PASSIVATION OF GOLD ELECTRODES WITH MCH

FUCTIONALIZATION OF SWNT WITH CROSS

LINKER

COVALENT IMMOBILIZATION OF ANTIBODY

BLOCKING OF NON-SPECIFIC SITES ON SWNT

SENSING OF ANTIGEN
AT DIFFERENT
CONCENTRATIONS

Dielectrophoresis

Fig: 1

Fig: 2

Dielectrophoresis
Specifications

Microfabricated gold electrodes are 3m apart

1.0l drop of SWNTs was used
An sinusoidal voltage of 1.5 V and 4MHz frequency at
temperature 3000c was applied

Passivation of gold electrode with MCH

6-mercapto-1-hexanol (MCH) is used to passivize the gold

electrode.
The gold microelectrode is insulated by MCH

Functionalization of SWCNT
This process is done to enhance the properties of CNT
Outer surface of CNT possess chemically inert environment
Two methods are generally used:
Non covalent
Covalent

stacking

Functionalization of SWCNT

Fig: Functionalization of SWCNTs with different methods

Functionalization of SWCNT
1-Pyrenebutaric acid N-Hydroxysuccinimide Ester (PyBt-NHS-Ester)

Immobilization of antibody
Is the imprisonment of cell , protein, enzyme etc. in a distinct support and
matrix.
Conventionally, there are Non-covalent and Covalent techniques generally
employed to immobilize antibodies

Immobilization of antibody

Fig: different methods of molecular Immobilization

Blocking of non specific sites

Material and Apparatus

1-Pyrenebutaric acid N-Hydroxysuccinimide Ester (PyBt-NHS-Ester) as
cross bi-linker
6-Methylcapto-1-hexanol (MCH) and Bovine serum albumin (BSA)
used as non specific sites blocking
Micromanipulator model 450PM-B probe station
C-reactive protein (CRP) as antibody antigen

Material and Apparatus

Micromanipulator model 450PM-B probe station

Results
I-V characteristics at different stages of modification

Current / A

SWNT
SWNT-PyBtNHS
SWNT-PyBtNHS/MCH
SWNT-Pyrenebutanamide-Anti CRP

-1
-0.5

Potential / V

0.5

Calibration graph

Equation

200

y =a +b

Adj. R-Squa 0.99818

R /R0 (% )

Value

Standard Err

Mean

Intercept -2.1994

3.10226

Mean

Slope

1.82883

48.905

150

100

50
1

Log[CRP] / ng mL-1

Conclusion

Fig : shows selectivity of CRP

Conclusion

Fig: Typical gate voltage dependence of the normalized source drain current (I sd)
at Vd=0.1V of SWNT

Conclusion
Anti-CRP was covalently immobilized on SWCNT channel by using bi-linker.
The specificity of the device was determined by exposing it to non-specific
biomarkers
The device showed linearity in the % change in resistance.
Thus, this high sensitivity and specificity together makes this device a better
technique
This technique provides prior detection of heart attacks, cancer at its early
stage and many more

Thank you