Medicinal Chemistry.

1
(Practical)
Assay Of Hyoscine Tablets
(Quantitative analysis)
Pharm. Dr. SULTAN ALKALEH

Assay of
Hyoscine

Assay Of Hyoscine

INTRODUCTION
o CHEMISTRY :
Scopolamine is atropane alkaloiddrugthat acts as a
competitive antagonist atmuscarinic acetylcholine
receptors;
it is thus classified as ananticholinergic,antimuscarinicdrug.

o ACTION AND USES

Works by suppressing and blocking muscle spasms in the gastrointestinal tract. It also has anticholinergic properties.

Indications for this medicine include abdominal pain and spasms in

the intestinal tract, especially when these symptoms occur as a result
of irritable bowel syndrome.

Patients have also taken it for bladder spasms.

Med.Chem.1 (Practical)

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Dr. SULTAN ALKALEH

Assay of
Hyoscine

Assay Of Hyoscine

Aim
o Determination of the amount of hysocine butyl bromide
present in a tablet dosage form in Yemeni pharmacys

Med.Chem.1 (Practical)

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Dr. SULTAN ALKALEH

Assay Of Hyoscine
Drug information:

o Generic Name:
Hyoscine butyl bromide 10mg. Antispasmodic
o Chemical Name:
..
o Chemical structure:
C21H30BrNO4
o M.wt:
360.4 g/mole.
o Pharmacopoeial limit content:
99-101 %.
Mfg Company information:
o Trade Name:
Busconil 10mg
Global Pharma Co.
o Mfg company:
Yemen
o Mfg country:
o Batch Number (B.NO):
Med.Chem.1 (Practical)

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Dr. SULTAN ALKALEH

Requirements. 5
Materials

Tools
Watch glass

Busconil 60mg

Spatula

Ethanol (50ml)

Balance

HCl 0.01 M (5ml)

Cylinder 100ml

Ph.ph indicator (3-4drops)

NaOH 0.1M (30ml)

Stir rod
Pipette
Burette
Burette stand
Conical flask

Funnel
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Dr. SULTAN ALKALEH

Requirements. 5
PRE-ASSAY
HCl 0.01 M

Step 1

Molarity of HCl

=
=

Step 2

0.01 M HCl

=
=

% x d x 10
M.wt

35x 1.16 g/ml x 10

36.46 g/mol

M required x V (ml)

Molarity of HCl
0.01 M x 100ml

11.13mol/l

Take 0.09 ml by pipette and complete the volume to 100ml by D.W

Med.Chem.1 (Practical)

= 11.13 mol/L

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Dr. SULTAN ALKALEH

= 0.09ml

Assay of
Hyoscine

Requirements. 5
PRE-ASSAY

NaOH 0.1M

M
0.1M

=
=

Wt =

Wt x 1000
M.Wt x V (ml)
Wt x 1000
40g/mol x 100ml

0.1M x 40g/mol x
100ml
1000ml

=0.4g

Dissolve 0.4g of NaOH in 100ml of D.W.

Ph.ph indicator indicator

Solution
Dissolve 0.1g of

Med.Chem.1 (Practical)

ph.ph in 80ml ethanol & dilute to 100ml by D.W

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Dr. SULTAN ALKALEH

Assay of
Hyoscine

Procedures

Procedures

1-Dissolve 60mgBusconilin mixture of (5ml

HCL 0.01M &50ml ethanol) .
NaOH 0.1M
2-Add 3-4 drops ph.ph indicator. (30ml)
3-Titrate with NaOH0.1 M when color change
stop & calculate the results.
4-Repeat the above steps without Busconil.
Ph.ph 3-4 drops
Hyoscine 60mg
HCl 0.01 M
(5ml)
Ethanol 50ml
Med.Chem.1 (Practical)

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Dr. SULTAN ALKALEH

Assay of
Hyoscine

Calculations. 7

Practical value:
Blank Sampl =.ml

Theoretical value:

1ml 0.1 NaOH 36.04 mg

Practicalhyoscine
value (ml) X

x =

Practical value (ml) x

36.04mg

1ml

% Hyoscine content:

% content

Med.Chem.1 (Practical)

Theoretical Value
Claim

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Dr. SULTAN ALKALEH

100

=.gm

........%