Beruflich Dokumente
Kultur Dokumente
16
Fig. 16-1
Fig. 16-5
Sugarphosphate
backbone
Nitrogenous
bases
5 end
Thymine (T)
Adenine (A)
Cytosine (C)
DNA nucleotide
Phosphate
Sugar (deoxyribose)
3 end
Guanine (G)
Fig. 16-7a
5 end
Hydrogen bond
3 end
1 nm
3.4 nm
3 end
0.34 nm
(a) Key features of DNA structure
5 end
(b) Partial chemical structure
Fig. 16-UN1
Fig. 16-8
Adenine (A)
Thymine (T)
Guanine (G)
Cytosine (C)
Fig. 16-9-3
(b) Separation of
strands
Fig. 16-10
Parent cell
(a) Conservative
model
(c) Dispersive
model
First
replication
Second
replication
Getting
Started
Replication begins at special sites called
origins of replication, where the two DNA
strands are separated, opening up a
replication bubble
A eukaryotic chromosome may have hundreds
or even thousands of origins of replication
Replication proceeds in both directions from
each origin, until the entire molecule is copied
Fig. 16-12a
Origin of
replication
Doublestranded
DNA molecule
Replication fork
Replication
bubble
0.5 m
Two
daughter
DNA
molecules
Fig. 16-12b
Bubble
Replication fork
Fig. 16-13
Primase
Single-strand binding
proteins
Topoisomerase
5
3
5
Helicase
RNA
primer
Fig. 16-14
New strand
5 end
Sugar
5 end
3 end
A
Base
Phosphate
Template strand
3 end
DNA polymerase
3 end
A
T
Pyrophosphate 3 end
C
Nucleoside
triphosphate
5 end
5 end
Antiparallel
Elongation
The antiparallel structure of the double helix
(two strands oriented in opposite directions)
affects replication
DNA polymerases add nucleotides only to the
free 3end of a growing strand; therefore, a
new DNA strand can elongate only in the
5to3direction
Along one template strand of DNA, the DNA
polymerase synthesizes a leading strand
continuously, moving toward the replication fork
Copyright 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings
Fig. 16-15a
Overview
Origin of replication
Leading strand
Lagging strand
Primer
Leading strand
Lagging strand
Overall directions
of replication
Fig. 16-15b
Origin of replication
3
5
RNA primer
Sliding clamp
3
5
Parental DNA
5
3
5
Fig. 16-16a
Overview
Origin of replication
Leading strand
Lagging strand
Lagging strand
2
Leading strand
Overall directions
of replication
Fig. 16-16b1
3
Template
strand
5
5
Fig. 16-16b2
3
Template
strand
5
5
RNA primer
5
Fig. 16-16b3
3
Template
strand
5
5
RNA primer
5
Okazaki
fragment
1
5
3
5
Fig. 16-16b4
5
5
Template
strand
RNA primer
5
3
5
Okazaki
fragment
3
3
3
5
Fig. 16-16b5
5
5
Template
strand
RNA primer
5
3
5
2
3
Okazaki
fragment
3
3
3
5
3
5
Fig. 16-16b6
5
5
Template
strand
RNA primer
5
3
5
3
5
Okazaki
fragment
3
3
3
5
3
5
Fig. 16-17
Overview
Origin of replication
Lagging strand
Leading strand
Leading strand
Lagging strand
Overall directions
of replication
Single-strand
binding protein
Helicase
5
3
Parental DNA
Leading strand
3
Primase
3
5
3 5
Lagging strand
DNA pol I
3
DNA ligase
1
3
5
Fig. 16-18
Nuclease
DNA
polymerase
DNA
ligase
Fig. 16-21a
Nucleosome
(10 nm in diameter)
DNA
double helix
(2 nm in diameter)
H1
Histones
Histones
Histone tail
Nucleosomes, or beads
on a string (10-nm fiber)
Fig. 16-21b
Chromatid
(700 nm)
30-nm fiber
Loops
Scaffold
300-nm fiber
Replicated
chromosome
(1,400 nm)
30-nm fiber
Looped domains
(300-nm fiber)
Metaphase
chromosome