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O2 CO2
OXYGEN
Sedikit larut dalam air: 3.2 mL O2 /1 L
blood plasma
Transporter: protein
hemoglobin
myoglobin
Hemoglobin (Hb)
Heme + Globin
Heme: porfirin dengan 4 bh cincin
pirol dan 1 Fe
Globin: protein
Hemoglobin - Myoglobin
Beda pada quaternary structure.
Hemoglobin : tetramer (2 )
Myoglobin : monomer (quaternary
structure tidak ada).
Myoglobin - oxygen lebih kuat dari
Hb
Perpindahan Oxygen sirkulasi ke cell
lebih mudah
Heme
Heme..
Hemoglobin
Heme-globin, (BM:68,000)
4 heme
porphyrin ring : Fe dan globin
Oxygen terikat dengan Fe ( fero)
Hemoglobin (HHgb) asam lemah (K = 1.4 x 10 ;
pKa = 7.85). Oxyhemoglobin (HHgbO 2) asam
lemah
-8
Oksi Hb Deoksi Hb
O2 meningkat pada alveoli: Rx
bergeser ke kanan dan ion H
meningkat
HHgb + O 2 <===> HgbO
2 + H+
Perbahan bentuk Hb
Fe moves in plane,
pulling proximal His
and Helix F
Dissosiasi OksiHb
,
1. Suhu : afiinitas O2-Hb
2. pH : afiinitas O2-Hb
Bohr Effect
Competition between oxygen and H +
the effect of pH and CO2 on the binding
and release of oxygen to Hb
Discovered by Christian Bohr
Binding of protons diminishes dioxygen
binding
Binding of dioxygen diminishes proton
binding
Important physiological significance
2,3,-DPG
Transport CO2
Larut dlm plasma (7 10%, )
Ion bicarbonat:
CO2+ H20 H2CO3 HCO3 + H ion
Hb sebagai pembawa O2
dan CO2
Fetal Hemoglobin
Chliride shift
Hypoxia
This is Fatal
Methemoglobin
Fe heme : feri (+3)
O2 tidak bisa diikat
Myoglobin
Small intracellular protein in
vertebrate muscle
Oxygen binding protein
Globular protein
First protein whose structure was
determined by X-ray crystallography
153 residues
8 helices
Heme is tightly wedged in a
hydrophobic pocket between the E
and F helices
myoglobin
Oxygen-binding curve of
myoglobin. Myoglobin is halfsaturated with O2
at an oxygen partial pressure
( pO2)
of 2.8 torr (dashed lines). The
hyperbolic shape of
myoglobins binding curve is
typical of the simple
binding of a small molecule to
a protein. The
background is shaded to
indicate the color change
that myoglobin undergoes as it
binds O2.
Hb sigmoidal (S-shaped
curve)
This permits the blood to
deliver much more O2 to
the tissue than if Hb had a
hyperbolic curve with the
same P50
Mb is a monomeric heme
protein
Mb polypeptide "cradles"
the heme group
Fe in Mb is Fe2+ - ferrous
iron - the form that binds
oxygen
Oxidation of Fe yields 3+
charge - ferric iron
-metmyoglobin does not
bind oxygen
Dioxygen binds as the
sixth ligand to Fe