Photoelectrochemical detection of

enzymatically generated CdS nanoparticles:

Application to development of immunoasssay

Barroso, J., L. Saa, et al. (2016). "Photoelectrochemical detection of enzymatically generated

CdS nanoparticles: Application to development of immunoassay." Biosensors and Bioelectronics
77: 323-329.

Delivered by: William

Aims
Shows feasibility application of photoelectrochemical setu
p by taking advantage of product coming from enzymatic
reactions to do biosensing process
Shows how incorporation of PEC cell and ELISA may imp
rove immunoassay
Cost reduction to perform immunoassay
How the cell woks?
In-situ generation of
semiconductor nanoparticles
Findings: interaction of Alkaline
phosphatase with phosphate substrate
lead to formation of H2S
H2S reacts with Cd2+ cations and form
CdS quantum dots (semiconductor
nanoparticles)

Detection of CdS nanoparticles

UV illumination release photons with
good amount of energy to excite
electrons within the nanoparticles
The SNPs (semiconductor
nanoparticles) will transfer electrons to
electrode
To perform better electron transfer
modification of electron surface was
made
 Scope of study / Experimental setup
Optimization of electrochemic
al setup
Electrode modification number
of CV cycles required
1-thioglycerol concentration
Potential difference applied

Study influence of thiophosph

ate & ALP concentration to cu
rrent response

Detection of antibody using m

odel system
Results

Max current
increase around
200M

Optimized TG
concentration =
600mM
Results
Detection of CdS SNP during
immunoassay

Detection limit of AB
Linear until 20
up to 2 ng/ml (S/N
ng/ml
=3)
Conclusion-Evaluation
Conclusion
Method of CdS QDs detec
tion (product of enzymatic
reaction) using PEC is pre
sented
The reported method coul
d reduce cost for immuno
assay test
Sensitivity of the reported
method was higher compa
red to conventional chrom
ogenic and fluorogenic as
say
Evaluation
Wiring may reduce
electrode cost. However
may also generate
unstable signal
Interesting concept, good
implementation of PEC
cell
Requirement to use
expensive microplate
reader was eliminated
Thank you for your attention