ANALYSIS LEVELS OF FATTY ACIDS FROM FREEZE-

DRIED
TERMITE QUEEN Macrotermes gilvus HAGEN.,
USING GC-MS
OrindiaAND ANTI-HYPERLIPIDEMIA
Suarmin, Lakmi Nurul Suci, Robby TEST
Kurniawan, Fuji Yasardi, Vivi Ramadhani and
Yohannes Alen*.
Advancing the Life Sciences and
Public Health Awareness (ALPHA) 2016
July 10-11, 2016
Nagoya, Japan

Faculty of Pharmacy, Andalas University, Campus Limau Manis, 25163 Padang,

 Introduction
Hypertension
Background Stroke
Migrain
Vertigo
Diabetes
(Fadil, 2015
Traditional and Shadiqul,
Protein
Carbohydrates usage 2014)
Crude Fiber
Fats Fatty
Acids
Anti-
- Monounsaturat Primary
ed Fatty Acids hyperlipide
Metabolite
(MUFA)
- Polyunsaturate
mia MUFA and PUFA
can decrease
d Fatty Acids LDL
(PUFA)
( J.N. Afiukawa, 2 increase HDL.
Recent Research
Burn Healing activity 0.5 g/10 g
(cream formula) (Alen, Y., et al.
2015)
Immunomodulator activity 10
mg/kg BW (Alen, Y., et al 2016)
Toxicity Acute and Sub Acute
(LD 50) > 1000 mg/KgBW (Alen, Y.,
et al 2016)
Based on that background
(introduction), the study needs to be
done to find out the constituent fatty
acids of Queen Termite Macrotermes
gilvus Hagen., Which was analyzed by
Using GC-MS and Anti-hyperlipidemia
test against animal Testing
3
Work scheme
Collecting and weighing samples

Frezee drying

Extraction and Fractionation

Analysis of the type of fatty acids by GC

MS

The Test of bioactivity

antihiperlipidemia

4 Data analysis
 ANIMALS TREATMENT GROUP TEST
Group Treatment
1 Induction hypercholesterolemia (negative control)
2 Induction hypercholesterolemia+ Simvastatin

(comparison)
3 Induction Hypercholesterolemia + 1 dose (75 mg /

KgBW)
4 Induction Hypercholesterolemia + 2 dose (150 mg /

KgBW)
5 Induction Hypercholesterolemia + 3 dose (300 mg /

KgBW)
Note: The test preparation were administered orally. 5
 Antihiperlipidemia Test

Preparation of serum: Serum was

The Test were
Blood was taken at analyzed for
conducted over 4
the orbital sinus,
Induction weeks, rats
blood was collected complete
Animal testing : blood were taken
hypercholesterole and then allowed to cholesterol levels
BW of male on days 0, 15
mia using High stand for 30 minutes. which were
albino rats 150 - and 30 days.
Centrifugation at
Fat Food (MLT) before taken consist of Levels
200 g, aged 2 - 3 3000 rpm for 20
and PTU 10 blood from rats, of total
months. minutes. The serum
mg/200 gBW. rats were fast for
was stored in the cholesterol, LDL,
18 hours before HDL, and
freezer with position
handling.
upright in a test tube. Triglycerides.

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 Research result
Sampling

50 head queen termite = 179.2410 Gram

 Research result
esults freeze drying

Weight powder = 33.4966 grams

Pemerian: fine powder, light cream
colored and distinctive odor
The yield = 18, 68% 8
Research result
Soxhletation Results of soxhletation
Process

The sample weight in

Soklet = 40 grams
Total Weight Oil = 28, 5647
grams
Research result
Identification of Samples Ket. Passed examine ethics

species: Macrotermes gilvus

FAMILY: Termitidae
Results of fatty acid analysis by GC-MS
Soxhletation
No. Parameter unit Result
mg / 100
1. Acid oleic 32812.70
g
mg / 100
2. linolenic acid 345.15
g
mg / 100
3. EPA 72.20
g
4. palmitic acid % 13.13
5. stearic acid % 7.42
Results of fatty acid analysis by GC-MS
Soxhletation

14%
Acid Oleic Linolenic acid

24% EPA Palmitic Acid

61%

0%
1%
Stearic Acid
Results of fatty acid analysis by GC-MS
Freeze dried
Powder
No. Parameter unit Result
mg / 100
1. Acid oleic 4357.20
g
mg / 100
2. linolenic acid 113.85
g
mg / 100
3. EPA 33.65
g
4. palmitic acid % 2.10
5. stearic acid % 1.00
Results of fatty acid analysis by GC-MS
Freeze dried
Powder

13%
Acid Oleic

Linolenic acid

28% EPA
57%

Palmitic Acid
0%
1%

Stearic Acid
Results of fatty acid analysis by GC-MS
Fresh Termite
Queen
No. Parameter unit Result
mg / 100
1. Oleic Acid 13709.70
g
mg / 100
2. linolenic acid 49.10
g
mg / 100
3. EPA 8.15
g
4. palmitic acid % 4.90
5. stearic acid % 2.83
Results of fatty acid analysis by GC-MS
Fresh Termite
Queen

13%
Acid Oleic Linolenic acid

23%
EPA Palmitic Acid

64%
0%

Stearic Acid
Table Comparison Of The Levels Of Fatty Acids

Soxhletation Freeze dried powder Fresh Termite Queen

 Average Profile Lipid levels SD (Standard Deviation) in each
treatment group before and after administration of the test
preparation (Mg / dl)
TC (mg / dl) TG (mg / dl) HDL (mg / dl) LDL (mg / dl)
N Befor Earl
o. Group e After Early End Early End y End
53.5 123.5 47.5 62 17 44 27 67.5
1 K- 5.71 4.76 3.81 3.8 2.23 3.31 5.43 5.70

K+
48.5 112.5 52.5 53.5 42.5 22 59
2 (Comparati 4:18 11.47 5.07 4.92
16 4
2.96 3.87 8.51
ve)
49.5 106.5 74 46.5 11.5 39 23 58.5
3 H1 3.87 9.07 20.71 3.57 3.20 4.63 3.16 4.21

43 68.5 10 38.5 24.5 46

4 H2 43 3.74 98 5.2
1.58 3.87 2.23 3.16 2.24 3.24

67.56 107.5 91 47 25 39.5 24.5 58.5

5 H3 6:06 7.97 28.87 9.03 3.08 4.66 8.47 2.65
Effect of Freeze-Drying Powders Queen Anai-Anai against,
Levels Hyperlipidemia Animal Test.
No TC (mg / TG (mg / HDL (mg / LDL (mg /
Test group
. dl) dl) dl) dl)
123.5 4.76 62 3.8 44 3.31 67.5 5.70
1. K- (70 ) (14.5 ) (27 ) (40.5 )
112.5 11.47 53.5 4.92 42.5 2.96 59 8.51
2. K+ (64 ) (1 ) (26.5 ) (37 )
106.5 9.07 46.5 3.57 39 4.63 58.5 4.21
3. H1 (57 ) (27.5 ) (27.5 ) (35.5 )
98 5.2 68.5 3.87 38.5 3.16 46 3.24
4. H2 (55 ) (25.5 ) (28.5 ) (21.5 )
107.5 7.97 47 9.03 39.5 4.66 48.5 2.65
5. H3 (40 ) (44 ) (14.5 ) (34 )
 Chart Average Profile Lipid levels SD (Standard Deviation) on each
group treatment before and after administration of the test preparation
(mg/dL)

K- Awal K- Akhir K+ Awal K+ Akhir H1 Awal

H1 Akhir H2 Awal H2 Akhir H3 Awal H3 Akhir
 Chart Average Difference Profile Lipid levels in each
treatment group after and before administration of the
test preparation (mg/dL)

TC (mg/dl) TG (mg/dl) HDL (mg/dl) LDL (mg/dl)

Information :
TC = Total Cholesterol
TG = Triglycerides
HDL = High Density Lipoprotein
LDL = Low Density Lipoprotein
K- = Test group without the test preparation, given PTU + MLT
K + = The comparison group using simvastatin
H1 = Test preparations with a dose of 75 mg / KgBW
H2 = Test preparations with a dose of 150 mg / KgBW
H3 = Test preparation at a dose of 300 mg / KgBW
Anova two way

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Test Duncan

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Conclusion
1. Omega-9 is an MUFA effects with lower LDL and raise HDL (Wood et al.,
1993). While omega-3 and omega-6 PUFA is that lower LDL at once. Queen
of termites is potentially as anti-hyperlipidemia due to the levels of the
omega-9 (oleic acid) 32812.70 mg/100 g (32.81%), while omega-3 (EPA)
72.20 mg/100 g (0.0722%) and omega-6 (linoleic acid) 345.15 mg/100 g
(0.345%).
2. The third dose the test groups showed the same effect in influencing lipid
levels i.e. the levels of total cholesterol, LDL, triglycerides and HDL. So the
increased dose not influence effect of lipid levels. So, use in small doses is
more advisable because of the fatty acids (omega-3, omega-6 and omega-
9) is a good supplement consumption, in the long run, it means either a
small or large doses provide the same effect. So that the public can use
the Queen of termites in small doses over a long time.
3. Queen of termites can be prepared daily as a supplement. Supplements
are part of the remedy.
Acknowledgements
Ministry Research, Technology and Education High
that already fund Research and research this in
Program PKM-PE 2015/2016,
Laboratory Pharmacology Staff, Faculty of
Pharmacy.
PT. Saraswanti Bogor
Thank you

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