Ecophile Co.

, Ltd
⊙ Characteristics

Items Our product Other product Remark

Harmony with native

Mix by hydrocarbon Mix by oil- microbes
1. Microbes mix
intake mechanism degrading capacity
(Environment-friendly )

Adding the microbes

2. Feasibility of Excellent feasibility in chronic
degrading metabolite,
- contaminated soil and
Adding the microbes lubricant contaminated soil
weathered oil
degrading lubricant

3. Condition of Adding the Adding general Excellence of initial oil-

large scale culture hydrocarbon nutrients degrading velocity
The products are officially certified by the MOE (Ministry of Environment) and KCG
(Korea Coast Guard) on efficiency & safety
 1.6
WLH-3

Emulsifying Activity (OD at 610nm)

1.4
DJ-3
WLH-1
1.2
WLH-2

1 CL180
DJ-1

0.8
IC-10

0.6 KH3-2
YS-7

0.4 WL-2

WL-1

0.2
DJ-2

0
0 20 40 60 80 100
Hydrophobicity (%)
 Group 1 Group 2 Group 3 Group 4 Uptake
Division Strains Characteristic
Mode
Group 1 80 WLH-2,3 Pseudo-
Group 1 DJ-3
EA , HP 
solubilized
Group 2 23 85
Group 2 WL-1, 2 EA , HP  Direct Contact
Group 3 84 80 41
Group 3 KH3-2, DJ-2 EA , HP  Solubilized
Group 4 90 88 91 93
WLH-1, DJ-1
Group 4 CL180, IC-10 MIXED MIXED
YS-7
 Mine ra liza t io n
Mine ra liza tio n
Bio
Bioma
mas ss s
CO + H2 O
CO2 2+ H2 O

HC
HC++OO
2
2

++N/N/PP++미생물
미생물

Fe e dba c k
Bio tra ns fo rma tio n Inhibitio n Re s idue s
Bio t ra ns fo rma t io n Re s idue s
(Ac ids , Ke to ns , (난분해성 :
(Ac ids , Ke to ns , (난분해성 :
Alcooho
Alc holsls..)..) Cyc
Cycliclics s, ,PAHs
PAHs..)..)
 IS
A) Initial B) After 18 days
ASP

SA AR IS

SA AR
 Measurement of total microbial
No.:
- Total microbial No :
Spread on the agar plate
Cultured on 30℃ for 3days
- Oil-degrading microbial No :
Cultured on 30℃ for 10days
After sampling, suspension
according to each dilution rate

Oil-degrading microbial No.:

Inoculated on 96 well micro
plate

Oil-degrading microbial No :
( 단위 : MPN/g-soil)
Oil-degrading activity is indicated by red color.
Total microbial No. :
Colony No.
(Units : CFU/g-soil)
Sample extraction Sample sonication Sample Cleaning Sample
concentration

Adding internal Sample Loading Data analysis Results

standard
 Developing solution: n-hexane
Primary developing
Aliphatic : 10cm developing

Secondary developing Developing solution : n-hexane : toluene (1:4)

Aromatic : 5cm developing

Developing solution :
Tertiary developing
dichloromethane:methanol(19:1)
Resin developing Fig. TLC/FID analysis of fractionated resin component
separated by modified method

(A) Sample roading, (B) Sample developing, (C) Evaporation of solution,

(D) Rod hold, (E) Analysis screen, (F) TLC/FID system
90
80
70
60
Diesel-contaminated soil
50
40 Crude oil-contaminated soil
30
Lubricant oil-contaminated soil
20
10
Crude oil-contaminated soil (the coast)
0

100
90
80
70 A Co. (Domestic)
60
F Co. (Foreign)
50
EcoGuard
40
30
20
10
0
Ga region Na region Da region
⊙ Technology development