Fig 8-12

cells grown cells grown

in 15N in 14N
Fig 8-21
hydroxyl group at 3
end of growing chain

deoxynucleotide
triphosphate
Fig 8-21
hydroxyl group at 3
end of growing chain

deoxynucleotide
triphosphate
Fig 8-21

new
phosphodiester
bond

pyrophosphate
(PPi)

this becomes new 3 end

 P

OH
P P P P P P P P P P P
OH

C T T C T C T
G G A A
G A A G A G A
C C T T

HO P
P P P P P P P P P P
 P

OH
P P P P P P P P P P P
OH

C T T C T C T
G G A A
G A A G A G A
C C T T

HO P
P P P P P P P P P P
 DNA polymerase (5'
(3' ->
-> 3'
5' polymerase)
exonuclease)

RNA primer P P PP PP P
P P
OH OHP OH
P P P P P P OH
OH P P
OH OH
OH OH

5' OH OH
OH OH OH

A
C C
C
C U U C C oops!
G G A
G A A G A G A
C C T T

3' HO
5'
P P P P P P P P P P P
 DNA polymerase (5' -> 3' exonuclease)
P
OH

new DNA OH RNA primer

P
P P P P P A P P P P P
OH
OH

OH OH OH OH OH OH

C T T C U C U
G G A A
G A A G A G A
C C T T

HO
P P P P P P P P P P P
 Fig 8-20

Replication Fork