Growth and Histopatological Changes of

African Catfish (Clarias gariepinus) was

addited by Probiotic of Bacillus in Rare
Medium after infected by Aeromonas
hydrophila

Angela Mariana Lusiastuti,

Mohammad Faizal Ulkhaq,
Widanarni
AQUACULTURE AND FISH HEALTH MANAGEMENT
UNIVERSITAS AIRLANGGA PDD BANYUWANGI
2016
 Introduction
Microorganism that can
modificate composition
of bacteria in digestive
Probiotic track, water and
sediment also as
biocontrol agent (Flores
2011).
Enzyme
Amino
Bacillus acid

Antibiotic
Spore
Ravi et al. (2007)
Luis-Villasenor et al.
(2011)
 Introduction
Preventif
Motile
Aeromona
s
Probiotic Septicemi
a
(MAS)

Resistenc Save,
e, Antibiotic ecofriendl
Residue y
Purpose
Evaluate the addition of a probiotic Bacillus on
rare medium through growth rate and
histopathological change in African Catfish
was infected by Aeromonas hydrophila.
 Probiotic
Growth
Ayoola et al. 2013
Essa et al. 2010
Noveirian and Nasrullahzadeh
2012
Histopatology
Sealey et al. 2010
Chelladurai et al. 2013
Nouh et al. 2009
Method
 Methode
In Vitro test:
Characterization of Bacteria
Morphology and
Biochemistry test
Production of Mutan Resistance
 Methode
In Vivo Test

Probiotik (CFUml -1)

A. hydrophila
Treatment Rif R (CFUml -1)

P4I1 RifR P4I2 RifR

P4I1 104 -
P4I2 - 104 103
Kom 104 104
K+ - -
K- - - -
 Method Probiotic
Design added once a
length 12,690,94 cm, weight 13,352,8 day (every
gram, density 30 fish morning)

Siphon
and
water
change

dimension 60x70x40 cm3,

water volume 40 liter A. hydrophila RifR added
in early rearing phase
Protein 36%,
twice a day
Parameter was
observed
Data Analysis

Growth rate ANOVA Duncan

test
Histopatologi
Water quality Descriptive
 Result
Growth rate
 Result
Histopathology
Treatm Pathology change
ent
Liver: leucosites infiltration (+), vacuolisation(+)
P4I1
Kidney: leucosites infitration (+), haemorrhagi (+).
Liver: leucosites infiltration(+), vacuolisation(+),
P4I2 haemorrhagi (+).
Kidney: leucosites infiltration (+), haemorrhagi (+).
Liver: leucosites infiltration (+), vacuolisation(+),
Kom haemorrhagi (+).
Kidney: leucosites infiltration (+), haemorrhagi (+).
Liver: leucosites infiltration (++), vacuolisation
epitel cell (+++), haemorrhagi (+).
K+
Kidney: leucosites infiltration (++), haemorrhagi (++
+), nekrose in kidney tubuli (++).
Liver: leucosites infiltration (+), vacuolisation (+)
K-
Ket: Kidney:
(+): foci leucosites
cell damaged, infiltration
(++): (+),
multifoci cell haemorrhagi (+)
damaged,
(+++): Difus cell damaged
 Result
Water quality
Parameter
Treatme Temper
nt Oksigen Amonia
ature pH
(ppm) (ppm)
(C)
0.01-
P4I1 2.72-4.6 26-28 5-7.5
0.03
0.01-
P4I2 2.7-5.4 26-27 5-7
0.03
2.18- 0.02-
Kom 26-27 5.5-7
5.98 0.04
0.01-
K+ 1.1-3.5 25-27 5-8
0.14
Conclusion
The addition of Bacillus ND2 RifR (104
CFU/mL) efective to increased the
spesific growth rate of African Catfish
and prevent the damage of liver and
kidney of African Catfish was infected
by Aeromoniasis.
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Thank you. .
.