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Ag/AgCl Ag/AgCl
Inner solution
Frit
analyte
Inner solution
Membrane (e.g. potassium,
sodium, pH, etc.)
Inner reference electrode/ inner
solution/membrane/analyte (external
solution)/external
reference electrode
Ion selective electrodes (ISEs)
A traditional pH measurement with a
glass electrode is the best known
potentiometric ion selective electrode
(ISE) (e.g. a thin glass layer with this
composition 22% Na2O, 6% CaO, 72%
SiO2)
There is no change in the inner solution
and there is no actual contact between
inner and outer solution for any
potentiometric probe or sensor
Contact with the solution is always
through the external reference electrode
(Luggin capillary)
Ion selective electrodes (ISEs)
AstheindicatorisonlyH+sensitive,andthepotentialofthereferenceisa
constant(becauseoftheconstantchlorideconcentrationintheelectrolyte),wehave
Ecell
Carbon dioxide sensor (3D)
silverspringcontact
Gaspermeablemembrane
Ir/IrOxelectrode
Ag/AgClelectrode
DuallumenPVCtube
Hydrogel
silverepoxy
epoxy
Carbon dioxide sensor (MEMS version)
A pH, CO2 and oxygen electrochemical
sensor array for in-vivo blood
measurements was made using MEMS
techniques
The pH and CO2 sensors are
potentiometric and the oxygen sensor is
amperometric (see further in this class)
The pH sensor is an ISE based on a pH
sensitive polymer membrane.
The CO2 sensor is based on an IrOx pH
sensor and a Ag/AgCl reference
electrode. .
Electrochemical oxygen sensor (fuel cell)
"Fuel cell" oxygen sensors consist of a diffusion barrier, a sensing
electrode (cathode) made of a noble metal such as gold or silver,
and a working electrode made of a metal such as lead or zinc
immersed in a basic electrolyt (such as a solution of potassium
hydroxide).
Immobilized antibody
Immobilized antibody
Oxygen sensor
Oxygen sensor
Immunosensors
Oygen is formed
Oxygen sensor
The oygen signal is lower the higher the amount of native antigen
From ISFET to ISNT FET
Homework
1. Design a combination glass electrode. Explain how it
works.
2. Design a planar immunosensor. How could you
incorporate a good reference?
3. Explain how a potentiometric CO2 sensor works.
4. List a list of reasons why the ISFET did not become a
commercial success.