THE ISOLATION AND CHARACTERIZATION OF ALKALOID

OF

RED BETEL LEAVES

Wulan Sari AS
Yuniar Candra P
Dhiya Alwan
Silvia Fidyati
Larissa Risky Amalia
 OUTLINE

Latar Belakang

Tinjauan Pustaka

Metedologi Penelitian

Hasil dan Pembahasan

Kesimpulan
BACKGROUND

Biological
Activities
 LITERATURE PREVIEW

Alkaloid

Daun Sirih Merah

Teknik Isolasi
 ALKALOID

(Sjamsul,1986)
RED BETEL LEAVES

Kingdom Plantae

Division Magnoliophy
ta
Class Magnoliopsi
da
Ordo Piperales

Family Piperaceae

Genus Piper
Species P. ornatum
 ISOLATION TECHNIQUE

Maserasi
Metanol
Ekstraksi N-
heksana

Metode
KLT
Fraksinasi Metode
KVC

Spektosko
pi UV-VIS
Karakteris Spektrosk
tik opi
Inframera
h
 FRACTINATION

KLT KVC
Mengetahu
i fase Fraksinasi
gerak yang setelah KLT
sesuai

Profil Fase gerak

Kromatogr dilihat dari
am hasil KLT
RESEARCH METHODOLOGY

Ekstraksi

Fraksinasi

Isolasi

Uji Kemurnian

Karakterisasi isolat
RESULTS AND DISCUSSION
Pict 1. TLC monitoring
chromatogram 3 faction. I
visually monitoring, II monitoring
under 254 nm UV-Lamp

Pict 2. TLC monitoring chromatogram. I

visually monitoring, II monitoring under 366
nm UV-Lamp
 Pict 3. TLC monitoring chromatogram (visually
monitoring)

Pict 4. TLC monitoring chromatogram fraction VLC

(FD : silica gel GF 254. FG : n-hexane solvent mixture :
ethyl acetate [7:3], spotting dragendroff look, neutral
fraction or alkaloids are weak bases, no. 2-9)
Pict 5. TLC monitoring
chromatogram (7), VLC fraction (FD :
silica gel GF 254, FG : n-hexane
solvent mixture : ethyl acetate [9:1],
spotting dragendroff look fraction no.
2, 67)

Pict 6. Chromatogram monitoring preparative TLC

(FD : silica gel GF 254, FG : n-hexane solvent mixture :
ethyl acetate [7:3], cicle sign spotting dragendroff
look)
 Pict 7. TLC monitoring
chromatogram single developer
(one dimention) under 254 nm UV-
Lamp (FD ; silica gel GF 254, FG :
solvent mixture
(a) n-hexane : Ethyl Acetate [8:2],
(b)Ethyl Acetate 100%,
(c) Methanol 100%)

Pict 8. 2 Dimention TLC

monitoring chromatogram
under 254 nm UV-Lamp (FD :
silica gel GF 254, FG : solvent
mixture
(a) n-hexane : ethyl acetate
[6:4]
 Pict 9. Result UV-Spectrum of visible light

Notes :
(a) Literature (Creswell and
friends, 2005 : 79)
(b) Literature (Shrinner and
friends, 2004 : 208-209)
 Pict 10. Result of the IR-
Spectrum
 SUMMARY
Based on research results, it can be
conclude that alkaloid compound has been
isolated with alifatic primary amine
compound neutral or weak bases.
Alkaloid isolation technique using
extraction, farctination, isolation, purity
test, and characteristic of isolates.
For this extraction using maseration with
two solvent is n-hexane and methanol.
 SUMMARY
For this fractination using TLC and VLC with
two solvent is n-hexane and ethyl acetate.
Isolates character using UV-VIS spectroscopy
and IR showed two bands at 3400 and 3500
showed a primary amine group, based on IR-
Spectrum it can be concluded that isolate
known that have alifatic primary amine
compound .
Result characterization of isolates with UV-
Visible spectrofotometry data showed that
isolates provide absorption 0,363