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[Purpos

With the release of nearly seven billion metric tons of carbon


dioxide each
Year and the overconsumption of fuel reserves, research for a
feasible alternative
fuel has intensified. Current possible alternatives, however,
have not reached
widespread commercialization. In light of this issue, the
scientific community has recently
taken interest in the unique structure of microalgae and the
malleability of metabolic pathways. The purpose of this study
[Hypothe
is]
Does nutrient stress have an
overall beneficial
impact on lipid accumulation?

While nutrient stress decreases growth


rate and photosynthesis, a significant
increase in the production of
[Method
Wrote concise research paper highlighting data relating
to the
metabolic structure of microalgae
Examined and analyzes various reviews and research
on microalgal biofuels
Collected research papers on the effect of stress on
microalgal biofuels
Examined research papers on prospects of microalgal
biofuels to narrow down focus
Studied scientific journals relating to biofuels in order
[Conclus
Nitrogen deprivation on microalgae n]
has led to a
general
increase in lipid accumulation through the
redirection of
Membrane lipids toward TAG production. Several
studies have shown
that growth rate is hindered due to the inability of
cells to produce the large amount of nitrogen-
containing membrane lipids necessary for growth.
recorded that production is better increased in
conjunction with other techniques
The limits in my research include the lack of access to
research due to the high number of papers requiring
payment. This has widely restricted access to useful,
potentially important articles that could help support
the hypothesis.
[Outcom
] me to
The Independent Research program has allowed
explore the
field I am interested in further without interference with
other classes.
Through this program, I have gained a deeper
understanding of microalgal
biofuels, the labwork conducted, and what I might
possibly do in the future.
s
In addition, I have learned that it is possible to produce
[Backgro
d]
Microalgal Biofuels- Fuels produced from the
products of
Microscopic algae
Triacylglycerol- Molecules made up of glycerol
and three fatty acids
Primarily used for energy storage
Lipid Accumulation- Production of lipids
Nutrient Stress- Incurring a reaction by
[Backgro
Some traits of microalgae:
d]
Account for a large portion of productivity in
marine
communities
Play significant role in the recirculation of carbon
in oceans
Rapid growth rate
Able to synthesize large amounts of TAGs and
[Results
[Backgro
Some forms of stress:
d]
pH conditions
Salinity
Temperature
Light
Nutrients (ex. Hydrogen peroxide,
nitrogen, phosphate, etc.)
[Produc
My research paper had a primary focus on nitrogen
deprivation as nitrogen plays an important role in
the metabolic
structure of microalgae.
I am submitting my paper to NHJS in order to help
inspire more students
to do research on microalgal biofuels in hopes that
more students may want to help research the
metabolic structure of microalgae. By doing so, we
Nitrogen deprivation has resulted in:
A significant increase in lipid accumulation
Decrease in membrane lipids
Decrease in photosynthetic capability
It has been suggested that, as nitrogen is in many essential
membrane lipids, microalgae turns lipid production toward
TAG production in order to conserve energy.
Nitrogen deprivation has shown:
27.5%-37.3% increase of neutral lipids in cell dry weight
in transgenic cells
Significant up-expression of genes relating to lipid
production
Upexpression of genes relation to carbon
fixation, hinting at increase capabilities
Hurdles in Production:
Requires large area of land
Crop protection costs
Extraction costs
Need higher productivity
Insufficient knowledge of metabolic structure

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