Beruflich Dokumente
Kultur Dokumente
Investigation in Sampang
District
Madura, East Java-
Indonesia
Farida D. Handayani
Ristiyanto
Mega Tyas
Abdul Haris
Misriyah
IVRCRD NIHRD-Directorate General of DC-EH
Ministry of Health, Republic of Indonesia
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IVRCRD SALATIGA
Institute for Vector and Reservoir Research
and Development
NIHRD-Ministry of Health
Vector borne diseases
and zoonosis control
Plague
Leptospirosis Nipah
Hantavirus Rabies
Malaria
Japanese encephalitis
Filariasis
Chikungunya
Dengue
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THE ORGANIZATIONAL STRUCTURE OF
NATIONAL INSTITUTE OF HEALTH RESEARCH AND DEVELOPMENT
NATIONAL INSTITUTE OF
HEALTH RESEARCH
AND DEVELOPMENT
(NIHRD)
SECRETARY OF NIHRD
CENTER FOR CENTER FOR APPLIED CENTER FOR CENTER FOR HUMANITIES,
BIOMEDICAL AND TECHNOLOGY OF HEALTH INTERVENTION POLICY, HEALTH,
BASIC TECHNOLOGY AND CLINICAL TECHNOLOGY OF AND PUBLIC
OF HEALTH EPIDEMIOLOGY PUBLIC HEALTH EMPOWERMENT
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Leptospirosis Studies (2004-
2015)
Leptospirosis Epidemiology
Reservoir
Agent of leptospirosis
Environmental Risk Factors
Leptospirosis Control
Rodent Control
Molecullar Epidemiology
Mollecullar Diagnosis
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6
Research sites:
Type of leptospirosis areas in
Indonesia
Highland areas
Rice Field
Flood Areas Area
Rice field
200-900 m
Slum Areas
50-200 m
sawah
25-50 m
025m
Environment, Host-Reservoir and Risk
Factors
Demak District Klaten Purworejo Kulonprogo,
Risk Factor District
Risk Factor Risk Factor
DIY
Risk Factor
Occupation related Housewife and Rice-field Farmers;
to the dirty places, domestic coconut-palm
Farmers
wetlands and workers (EI, plantation
rivers; 2005) Reservoir farmer
Rat infestation Sugar cane R. tanezumi
Reservoir
plantation Serovar
R. tiomanicus
Reservoir farmer Autumnalis
Serovar
R.norvegicus, R. Reservoir
Icterohaemorrh
tanezumi dan R. R. tanezumi agia dan
exulans
Serovar Bataviaea
Serovar Semak/ hutan sekunder
Autumnalis &
Autumnalis & L. Icterohaemorrha
sawah (Kec. Nanggulan, D.I Yogyakarta ;
Icterohaemorrhagi giae dan
tinggi/pegunungan
pegunungan 200-400 m)
Ekosistem datran
ae dan Bataviaea pemukiman
Bataviaea (Kec. Jogonalan, Kab. Klaten
dan Kec. Purworejo, Kab.
pantai sawah
Purworejo, Jawa Tengah;
(Kec. Bonang, Kab. Demak; dataran rendah, 50-200 m, )
daerah pantai, 0-50 m)
Leptospirosis Outbreak in
Sampang District? (Madura-
East Java)
Leptospirosis
Cases Decline
Screening of
Leptospirosis
1. Human
1. Human 2. Reservoir
2. Reservoir
3.Environme
3.Environme
nt
nt
Mapping of
Mapping of leptospiosis
leptospiosis
Spatial
Spatial
analysis
analysis
Intervention
Intervention
1.
1. 2.
2.
Community Environment
Community Environment
3. Rodent
3. Rodent control
control
Coordination
Provincial level - District level- community level
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Screening Results in
Humans
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Rapid diagnostic test
Detection of pathogenic leptospires in
human cases
Hasil RDT Merk Hasil RDT Merk
Hasil PCR SD Focus Keterangan
Lane1 m : Marker
Lane2 K+ : Kultur lepto Positive
Lane3 M1 : Choirul Umam Human blood Negative negative Positive Sampel darah terlalu sedikit
Lane4 M2 : Bahri Human blood Negative - Negative
Lane5 M3 : M.ali Human blood Positive - Positive
Lane6 M4 : Moh.subhan Human blood Positive Positive tidak dilakukan
Lane7 M5 : Moh.Aditya Human blood Positive - Negative
Lane8 M6 : Fauziah Human blood Positive - Negative
Lane9 M7 : Sugianto Human blood Positive Positive Positive
Lane10 M8 : Khoirunisa Human blood Positive - Negative
Lane11 M9 : Tuma Human blood Positive Positive Negative
Lane12 M10 : Namartatula Human blood Positive - Positive
Lane13 M11 : Leyza Tara Human blood Positive - Positive
Lane14 M12 : Hanaf Human blood Negative - Negative
Lane15 M13 : Fathorahman Human blood Positive - Positive
lane 16 M14 : Abd.Aziz Human blood Positive - Negative
Lane17 M15 : M.abdullah Human blood Positive - Negative
Lane18 M16 : Syamsiyah Human blood Positive Positive Positive
Lane19 M17 : Murawati Human blood Positive negative Negative istri pasien atas nama ali
Lane20 M18 : Rahmat N Human blood Positive Positive tidak dilakukan
Lane21 M19 : Sahdi Human blood Positive - Positive
Lane22 M20 : Ach.Animan Human blood Positive - Positive
Lane23 M21 : Sugiarto Human blood Positive - Positive
Lane24 M22 : Chitamul Human blood Positive tidak dilakukan Positive Awal MRS dengan diagnosa DBD
Lane25 K- : Kontrol Negative Negative
Lane26 K- : Kontrol Negative Negative
Leptospirosis laboratory (IVRCRD
Salatiga)
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Storage of Serovar
Culturing 20 serovars
MAT (Microscopic Agglutination Test)
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Clinical symtoms of
Leptospirosis cases 1. Fever
2. Contact with flood
water
3. Headache
4. Nausea
5. Myalgia
6. Ikterik
7. Brown urin
8. Cough
9. Asphyxiate
10. Conjuntivitis
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Laboratory test from Sampang
Hospital
1. SGOT
2. Kreatinin
3. SGPT
4. Hiperglikemia
5. Hiperbilirubinemia
6. High urea in
blood
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Distribution and
CFR of
leptospirosis
cases in
Sampang
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Environmental investigation and
intervention
Environment surveys in Sampang, East Java
1. Chlorine survey in the water reservoir
Chlor content Chlor measurements * (mg/L)
Sub requirement for
Distric killing Water container Puddle/Drain
t Leptospira sp Pre Post Pre Post Well
(mg/L)
Ron Tengah 0 1.23 0 0,5 Changing Chlor levels at
Gn. Sekar 0.5 3 0,25 1.90 0 0,25 pre and post application of
Kontrol 0 0 0 0 Sodium Hypochlorite.
(Dalpinang)
2. Disinfectant (Sodium
hypochlorite)
Water container in Ron Tengah
was positive bacteria
pathogenic Leptospira
Water examination after
intervention of Sodium
Hypochlorite was negative
Administration of Disinfectants Sodium
Hypochlorite 1%
Water sampling in
river
Giving disinfectants in
public
Percentage of population receiving and using
sodium hypochlorite in Sampang district,
East Java
(2 villages; n = 150 samples)
Pengujian sodium hipoklorit thd
tumbuhan di Lab. B2P2VRP
Domestic rodent R.
tanezumi (40%) and R.
norvegicus (36%) were
dominant than other
species
Treatments Control
Handed poster to
Pemasangan
baliho
Penyuluhan
Health Promotion
leptospirosis prevention
materials
Leptospirosis Etiology
Cause Baliho
Transmission pathway
Symptoms
Reservoir
Perlakuan Kontrol
(Kel.Ron Tengah & Gn. (Kel. Dalpinang)
Sekar) N=55
N=45 (Ron) ; 57 (Gn.Sekar) Perlakuan
TINGKAT PENDIDIKAN
Kontrol
There was a transtition of knowledge of leptospirosis
prevention before and after the health promotion intervention
(p<0,5).
Health Promotion Method which was
preferred by respondents in Sampang, Jawa
Timur