DIAGNOSTIC FUNDAMENTALS OF

CLINICAL MICROBIOLOGY
 RIGHT THERAPY

RIGHT DIAGNOSIS
OF INFECTIOUS
DISEASE

CONTROL
 CLINIC RADIOLOGY

RIGHT DIAGNOSIS OF
INFECTIOUS DISEASE

LABORATORY DIAGNOSIS

P.A. MICROBIOLOGY P.K.

 RIGHT METHOD
SPECIMEN Specific
Sensitive

RIGHT MICROBIOLOGY
DIAGNOSIS

EQUIPMENT HUMAN RESOURCE

/REAGENTS Trained
Tested Highly dedicated i
 SPECIMEN
GOOD
Right material
- Right collection time
- Right collection location
- Right collection procedure
- Right storage and transportation
- Right quantity
 SPECIMEN COLLECTION
1. PROCEDURE
Sterile : collection location, collection tool, container
2. TIME
Before antibiotic therapy
In certain stadium of disease where microbial agents of disease
could be found.
tifoid : Salmonella isolation from blood firs mg of disease
furunkel: mature, or at incision
In the mornings, collected at 24 h
3. LOCATION
Where causative agent would probably be found : most bad,
most deep
paling dalam
4. AMOUNT : according to necessity.
 SPECIMEN STORAGE
Container: sterile dan sealable (screw
capped bottle).
Storage time: must be immediately
processed, may not exceed 24 hours.
Temperature : depends on suspected
bacteria : 4oC, room temperature.
If necessary: transport medium
 SPECIMEN TRANSPORTATION
Must be in suitable environment for the
suspected bacteria.
- transport medium
- Room temperature /thermos bottle with
ice inside
Container may not be carried with bare
hands.
Long distance : CONTAINS
MATERIAL THAT CAUSE DISEASE.
IDENTIFIKASI BAKTERIOLOGIS
SPECIMEN
BACTERIA MACROMOLECULE

Bottle/ Ag Ab DNA
Direct
preparation transport med.

Microskopic Isolation
A. Pemeriksaan Mikroskopis
SPECIMEN

DIRECT
PREPARATION

WET MOUNT STAINING

G RAM ACID FAST FLUORESCENCE

 Preparat langsung
1. Wet Mount
- morphology
- movement
2. Stained preparation
a. Gram: morphology, Gram characteristic
b. Acid fast: morphology, acid fast
characteristic
c. Fluorescence: me sensitivity
 BIAKAN BAKTERI

SPECIMEN
BOTTLE/ TRANSPORT MED.

ENRICHMENT MEDIUM

SELECTIVE/DIFFERENTIAL MED

BIAKAN MURNI

Identifi-
cation GRAM TES BK AG DNA Tes R
 Medium
1. Transport medium = carrier medium
2. Basic medium
3. Enriched medium
4. Enrichment medium
5. Selective medium
6. Differential medium
7. Special medium
8. Anaerobic medium
Medium :
artificial environment used to maintain life
and to culture bacteria outside the host.
Colony :
mass of growing bacteria on solid medium

Isolated colony:
separated colony, decendant of a single
bacterial cell.
Inoculating on solid media