BIOCHEMISTRY

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Metabolic Pathways

Presented by:

ROBERT DOMINIC D. GONZALES

STC REVIEW CHAIRPERSON
2016-2017
 #1
This serves as the central
pathway or central hub of the
biochemical pathways
happening within organisms.

Answer: Krebs Cycle

 #2
Glycolysis is a/an____ reaction.
a) Catabolic
b) Anabolic
c) Both A and B
d) None of the above.

Answer: (A) Catabolic

 #3
The first stage of glucose
metabolism in organisms from
bacteria to humans. This is also
the first biochemical pathway
elucidated.

Answer: Glycolysis
 #4
In glycolysis, STEP 8 involves
the conversion of 3-
phosphoglycerate to 2-
phosphoglycerate. Name the
ENZYME and REACTION
involved.

Answer:
ENZYME: Phosphoglyceromutase
REACTION: Isomerization
 #5
In glycolysis, this ENZYME
facilitates the conversion of
Dihydroxyacetone phosphate
(DHAP) to Glyceraldehyde-3-
phosphate (G-3-P).

Answer:
Triose phosphate isomerase
 #6
The shuttle mechanism
occurring in the MUSCLES
and BRAIN.

Answer:
Glycerol-Phosphate Shuttle
 #7
The shuttle mechanism
occurring in the HEART,
LIVER, and KIDNEYS.

Answer:
Malate-Aspartate Shuttle
 #8
Carbohydrates are also termed
to as __________ and
_________.

Answer:
polyhydroxyaldehydes
and polyhydroxyketones
 #9
In Beta-Oxidation of Fatty
Acids, the enzyme THIOLASE
catalyzes this REACTION of
the Beta-ketoacyl-CoA. What is
this reaction?

Answer:
Cleaving/Cleavage
 #10
These are enzymes which
hydrolyze lipids.

Answer:
Lipases
 #11
Complete the pairing below.

CARBOHYDRATES: GLYCOLYSIS
____________: BETA-OXIDATION

Answer:
LIPIDS/FATS/FATTY ACIDS
 #12
The main pathway for the
catabolism of fatty acids.

Answer:
Beta-Oxidation
 #13
TRUE OR FALSE
The Citric Acid Cycle is purely
catabolic.

Answer:
FALSE
 #14
The Citric Acid Cycle is also
known as ___________.

Answer:
Krebs Cycle, TCA Cycle,
Tricarboxylic Acid Cycle
 #15
The energy pay-off phase
happens during which steps of
glycolysis?

Answer:
Steps 6-10
 #16
This type of metabolism if
observed in the fermentation
and formation of alcohol and
lactic acid (lactate). A condition
where there is ABSENCE OF
OXYGEN.

Answer:
Anaerobic Metabolism
 #17
The IRREVERSIBLE STEPS of
Glycolysis.

Answer:
Steps 1, 3, and 10.
 #18
The formation of glucose from
non-carbohydrate source (e.g.
amino acids, fatty acids).

Answer:
Gluconeogenesis
 #19
Which is a more energy-
efficient and reliable shuttle
mechanism?

Answer:
Malate-Aspartate Shuttle
 #20
Hydration reactions are
observed in steps 1 and 7 of
the Krebs Cycle. What are the
substrates involved in this
reaction?

Answer:
Oxaloacetate + Acetyl-CoA Citrate
Fumarate Malate
PRACTICE
QUESTIONS ON ATP
ACCOUNTING
Account for the NET ATP yield of the
following reactions:

Note: via GLYCEROL-PHOSPHATE

SHUTTLE ANSWERS:

#1) glucose lactate (#1) 2 ATPs

#2) fructose-1,6-bisphosphate pyruvate (#2) 7 ATPs

#3) acetyl-CoA CO2 + H2O (#3) 10 ATPs

#4) maltose CO2 + H2O (#4) 60 ATPs

#5) palmitic acid CO2 + H2O (#5) 106 ATPs

Account for the NET ATP yield of the
following reactions:

Note: via MALATE-ASPARTATE

SHUTTLE ANSWERS:

#1) phosphoenolpyruvate Acetyl-CoA (#1) 3.5 ATPs

#2) lauric acid CO2 + H2O (#2) 78 ATPs

#3) glycogen lactate (#3) 3 ATPs

#4) isocitrate malate (#4) 7.5 ATPs

#5) lactose CO2 + H2O (#5) 64 ATPs

Potato corner is known to house one of the best
French fries in the countrythe universe, rather.
Their french fries are cooked in coconut oil.

NOTE:
1 French fry contains 10 moles of glucose.

Coconut oil contains 3 moles of palmitic acid, 2

moles of lauric acid, and 1 mole of myristic
acid.

QUESTION:

Calculate the NET ATP yield of 1

French fry from Potato Corner. ANSWER:
886 ATP
Thank you for joining me on
my last tutorials. Kuya Robs
gonna miss you all! Do
great on your exams!
{ God bless you always!