Beruflich Dokumente
Kultur Dokumente
3. Mechanism of chaperone
Method A B
pG-KJE8
Figure 2. Chaperone cycle of (A) GroEL and GroES, and (B) DnaK, DnaJ, and
GrpE.
Expression
SDS-PAGE
Conclusions
Results
The solubilisation of the rhPT2 fusion intein-tag was achieved by
chaperone modulation. These results revealed that chaperones are
Results tools for protein folding and assists the solubilisation process of
rhPT2 in E. coli.
1. Cloning of rhPT2 into pTWIN1
A B
Acknowledgments
6000 M 1 2 pTWIN1 6000
M 1 pTWIN1
2
This work is supported by grant Penelitian Unggulan Strategis
Nasional (PUSNAS) from Directorate of Higher Education,
Ministry of National Education, Indonesia.
1000 1000
rhPT2 (thiol) rhPT2 (pH)
References
Figure 1. Cloning of (A) rhPT2 (thiol) and (B) rhPT2 (pH) into pTWIN1. M Soejima et al. 2001. J Biochem. 130, 269-277
is marker, 1. undigested plasmid and 2. double- Strocchi et al. 2006. Proteomics. 6, 193-206
digested plasmid resulting pTWIN1 and rhPT2. Walter and Buchner. 2002. Angew. Chem. Int. Ed. 41, 1098-1113