Beruflich Dokumente
Kultur Dokumente
SEM 2 2006/2017
PREPARED BY
HJH ROSLINDA SESWOYA
General Requirements
1. Be properly prepared to do the experiment scheduled for that day.
2. There will be no smoking, using CELL PHONES, drinking, chewing gum, or eating
in the laboratory.
3. Do not touch your face, handle contact lenses, apply cosmetics or put your
fingers in your mouth.
4. Always wash your hands after handling viable material.
5. Avoid working alone in the laboratory.
6. Horseplay will not be tolerated. Avoid distracting others that are working.
7. At the end of each lab session, clean your lab bench, put away all materials,
and wash your hands thoroughly before leaving the laboratory. Wastes should
be deposited in appropriate receptacles.
8. Gloves and lab coats are to be removed prior to leaving the laboratory and may
not be worn in non-laboratory areas.
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Personal Protective Equipment (PPE):
1. Observe all laboratory signs and chemical labels for required protective
equipment.
2. Safety glasses or chemical splash goggles will be worn at all times when
performing laboratory work.
3. Wearing contact lenses is discouraged.
4. Always wear gloves when handling any chemicals or microorganisms.
5. You are required to wear laboratory coats while working to prevent
contaminating your clothes.
6. Wear shoes that cover the entire foot; NO sandals or open shoes.
7. Tie back long hair and confine loose clothing to keep away from flames
and chemicals.
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Hazardous Material Safety:
1. Unauthorized experiments are prohibited. Perform the experiments as directed by your instructor.
2. Never take chemicals, supplies, or equipment out of the laboratory.
3. Students are not allowed to enter the Chemical Stockrooms.
4. Know where the MSDS (Material Safety Data Sheets) are for your chemicals. MSDS contain information
that you will need in case there is an emergency.
5. Never taste or smell a chemical.
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MA01 Total Suspended Solid (TSS):
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MA01 Total Suspended Solid (TSS):
Weigh the Pour 15 mL of water
Switch on record the sample ( from UTHM
the balance initial dry lake) on the filter paper
( at least 30 weight of and leave it until the Take the filter paper,
min before filter paper filtration finishes place on the evaporating
the test) dish
Weigh and
record the
final dry Place the evaporating
weight of dish to cool at room
filter paper temperature in Place the evaporating
dessicator disk inside the drying
oven at 1050C for at least
1 hour or until
completely dry.
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MA01 Total Suspended Solid (TSS):
For Environmental Engineering Laboratory ( BFC 32501) SEM 2 2016/2017, submit
the lab report by answering the following
B) Discussion
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MA02 Bacteria Count
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MA02 Bacteria Count
Note Prepare freshly by boiling the
All equipment used mixture of:
in this experiments peptone = 5 g
should be sterilized Inoculate
plate
Beef extract = 3 g
in an autoclave at containing Agar = 15 g
1210C for 15 min nutrient agar Distilled water = 600 mL
Carefull with ethanol
and Bunsen burner
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MA02 Bacteria Count
For Environmental Engineering Laboratory ( BFC 32501) SEM 2 2016/2017, submit the
lab report by answering the following
B) Discussion
1) State the common bacteria indicator used for monitoring water quality and explain
why bacteria indicator is important in water management 11
MA03 Biochemical Oxygen Demand (BOD)
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MA03 Biochemical Oxygen Demand (BOD)
1. Collect water from UTHM lake
2. Measure the pH-
i) if the pH is in the range of 6.5 to 7.5- proceed to Step 3
step 3
ii) if the pH of sample is less than 6.5, then use base to improve the
pH to 7, refer to standard method for detail procedure Seeded BOD
iii) if the pH of sample is bigger than 7.5, then used acid to improve the pH to
pH to 7, refer to standard method for detail procedure Seeded BOD
3. Using the details as below, determine the sample size using Eqn A and
Eqn B
a) Based on the literature, the BOD of A = 400 mg/L ( subject to changed )
b) Assume the DO of saturated dilution water = 8 mg/L
c) The DO depletion at the end of 5 days incubation = 2 mg/L
d) The residual DO = 1 mg/L
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MA03 Biochemical Oxygen Demand (BOD)
Eqn A = minimum waste to be filled in BOD bottle
= [(2 mg/L ) X 300 mL] / 400 mg/L
= 1.5 mL
Eqn B = maximum waste to be filled in BOD bottle
= [(8 mg/L- 1 mg/L ) X 300 mL] / 400 mg/L
= 5.2 mL
Therefore sample size = 1.5 to 5.2 mL (Let say choose 3 mL)
4. Take 5 BOD bottles. Use 3 bottles for sample, and 2 for blank
5. Fill the BOD bottles ( one by one) as below:
Di = initial DO ( mg/L)
Df = final DO (mg/L) , 5 days or 3 days
P = Volume of sample used / Total volume
Note:
1) DO in blanks should not deplete more than 0.2 mg/L
2) DO in sample should not less than 2 mg/L
3) Final DO in the samples should not less than 1 mg/L
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MA03 Biochemical Oxygen Demand (BOD)
For Environmental Engineering Laboratory ( BFC 32501) SEM 1 2016/2017, submit
the lab report by answering the following
Sample 1
Sample 2
Sample 3
Blank 1
Blank 2
B) Discussion
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GOOD LUCK !!!
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