METHODOLOGY

STREAK PLATE PROCEDURE

1. Sterilize
inoculating loop

BAP

MAC

4) Incubate at 35⁰-37⁰C for 18-

2) Get an unknown 24 hours
sample from the test
tube to be tested 3) Perform isolation streaking for MAC
using a sterile agar.
inoculating loop.
 METHYL RED TEST
BIOCHEMICAL TESTS METHODS 3) Slightly stab the
center of the
medium using a
straight inoculating
needle containing
the unknown
organism.
VOGES-PROSKAUER
TEST
4) Slightly stab the
middle of the
medium, using a
straight inoculating
needle containing the
unknown organism.
1) Heat first the 2) Pick a small amount of
INDOLE TEST
inoculating needle. unknown organism grown
on MAC. 5) Using a sterile
inoculating needle
containing the
unknown organism,
stab slightly the
center of the medium.
 BIOCHEMICAL TESTS METHODS UREASE TEST
8) Using a straight
inoculating needle
containing the
unknown organism,
stab the center of
the medium to the
bottom of the tube
and then streak the
slant.

TRIPLE SUGAR
IRON AGAR
9) Stab the center
6) Sterilize the 7) Get enough amount of of the medium to
inoculating needle. unknown organism grown the bottom of the
on MAC. tube, using an
inoculating needle
and then streak the
surface of the slant.
 CITRATE AGAR TEST
BIOCHEMICAL TESTS METHODS 12) Stab lightly the
center of the
medium using the
inoculating needle
containing unknown
organism.

LYSINE IRON AGAR

13) Stab the center of
the medium and
streak the surface of
urea agar slant with
the isolated colony,
10) Sterilize the 11) Pick a small amount of using inoculating
inoculating needle unknown organism grown needle.
first. on MAC.

14) Incubate all media at

35⁰-37⁰C for about 18-24
hours.