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3 RESULTS 6 ACKNOWLEDGEMENTS
I. Introduction
3
Preeclampsia (PE), occurs in 3-5% of pregnant women, the leading causes
of maternal and perinatal mortality. PE is considered to occur following a
two-step process.
sequenced on
The synthesized cDNA (3 ml) was
the NextSeq used as a template for realtime
500 platform. PCR using a forward primer
specific for the studied RNA
A logistic regression
analyse the relationship between the miRNA levels and the
probability of PE development.
miR-423-5p could clearly differentiate the early-onset PE group from the control group at 11-13 GW although the
manifestation of PE clinical signs only occurred at 28-33 GW.
Identification of Potential Early Biomarkers of
Preeclampsia
III. Results
Identification of signalling pathways potentially regulated by the associated PE miRNAs 24
processing
and
presentation
of antigen
cell apoptosis
graft-versus-
and DNA
host disease
repair
Potential
regulators
miR-532-5p, -423-
5p, -127-3p, -539-
5p, -519a-3p, and - complement
controlling 629-5p and let-7c- and
the cell cycle 5p coagulation
cascades
reninangioten
focal
sin system,
adhesion and
progesterone
p53-mediated
-mediated
signalling
oocyte
pathway
maturation
↑ hypoxia/ischaemia
in the placenta ↑ expressed C14MC
miRNAs in trophoblast
→changes in the cells during the 1st
expression of miRNAs
trimester, ↑ C19MC
due to ↑ trophoblast miRNAs in the 3rd
cell shedding and trimester
necrosis.
Preeclampsia
Identification of Potential Early Biomarkers of
Preeclampsia
IV. Discussion
Factors associated with PE 29
miR-127-3p is the miR-539-5p in
main mitochondrial let-7 in cytotrophoblast
posttranscriptional fragmentation and proliferation
regulator cell apoptosis
miR-423-5p in
inhibiting miR-629-5p in cell
miR532 in thrombosis
progesterone motility and invasion
receptor transcription