Beruflich Dokumente
Kultur Dokumente
General Concepts
Signs
Symptoms
Etiology
Epidemiology
Pathogenicity
Virulence
Photos used from various UF/IFAS Extension Publications or provided by UF/IFAS faculty and staff, unless otherwise stated.
Symptom of Disease
• observation of how the host is
manifesting infection by a pathogen
and disease development due to a
pathogen
University of Florida - IFAS 2
Signs vs. Symptoms
Signs of Disease
Fungal mycelia on roots (microscopic view) Pythium oospores on roots (microscopic view)
Mosaic
Ringspot
University of Florida - IFAS 16
Plant Pathology Terms
• Pathogenicity: the pathogen either does
or does not cause a disease; a question
with yes or no answer
• Virulence: relative capacity of pathogen to
cause disease; range from minimal damage
to dead plant
• Etiolgy: determining the cause of disease
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Etiology and Pathogenicity Testing
1) Consistent isolation of a pathogen from
symptomatic host tissue
2) Pathogen is grown in pure culture and its
characteristics documented
3) Inoculation of a healthy plant with the pure
culture of the pathogen, and inoculated plant
must then develop symptoms similar to those
observed initially
4) Recovery of the same pathogen used for
inoculation purposes
This is general scheme; non-culturable plant pathogens have special rules.
University of Florida - IFAS 18
Etiology and Pathogenicity Testing
Example: Fusarium Wilt of Queen Palm
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Control F. oxysporum
F. incarnatum-equiseti F. proliferatum
University of Florida - IFAS 21
Plant Pathology Terms
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How do pathogens enter plant?
•Viruses and Viroids and Fastidious Bacteria
most require vectors; a few mechanical entry
•Bacteria – most enter through natural
openings or wounds
•Fungi & Oomycetes – enter through natural
openings, wounds; by mechanical pressure
or enzymes they produce; a few by vectors
•Nematodes – stylets used to gain entry
Plant Epidermis
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How do pathogens enter plant?
Leafhopper
(insect vector)
Use mouth parts to
penetrate
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How do pathogens enter plant?
Leafhopper Bacteria
(insect vector) on water film,
Use mouth parts to enter
penetrate through stoma
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How do pathogens enter plant?
Leafhopper Bacteria
(insect vector) on water film, Fungal Spore
Use mouth parts to enter entering
penetrate through stoma through stoma
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How do pathogens enter plant?
Leafhopper Bacteria Appressorium
(insect vector) on water film, fungal structure from Fungal Spore
Use mouth parts to enter spore for direct entering
penetrate through stoma penetration through stoma
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How do pathogens enter plant?
Leafhopper Bacteria Appressorium
(insect vector) on water film, fungal structure from Fungal Spore
Use mouth parts to enter spore for direct entering directly
penetrate through stoma penetration through stoma
Fungal Hyphae
can grow between
cells or penetrate cells
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Disease Development
• pathogen comes in contact with plant
Susceptible
Host
Favorable DISEASE
Environment
Pathogen
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Disease Development
Susceptible
Nematode Host Fungus
W. Deacon, Univ. of Edinburgh
Favorable Pathogen
Environment
Mite Insect
USDA/ARS
University of Florida - IFAS 32
How do plant pathogens move
from plant to plant if they are
not moved by a vector?
• Wind dispersal of spores
• Splash dispersal of spores – rain, irrigation
• Physical movement of soil-borne pathogens
that don’t produce spores – ex: soil tillage
• Nematodes swim or move with soil
• Seed associated – internal or external
• Plant associated – cuttings, grafting