

109.9 – 121.9
97.8 – 109.8
85.7 – 97.7
Leaf Length Category
(Class Interval) 73.6 – 85.6
61.5 – 73.5
49.4 – 61.4
37.3 – 49.3
25.2 – 37.2
13.1 – 25.1
1.0 – 13.0

0 5 10 15 20 25 30 35 40
Relative Frequency (%)
Figure 1. The age structure of unfertilized Coleus blumei
 109.9 – 121.9
97.8 – 109.8
85.7 – 97.7
Leaf Length Category
(Class Interval) 73.6 – 85.6
61.5 – 73.5
49.4 – 61.4
37.3 – 49.3
25.2 – 37.2
13.1 – 25.1
1.0 – 13.0
0 5 10 15 20 25 30 35 40 45
Relative Frequency (%)
Figure 2. The age structure of fertilized Coleus
blumei
 Age structure of Coleus blumei
109.9 – 121.9 Fertilized
97.8 – 109.8
Leaf Length (mm)

Unfertilized
85.7 – 97.7
73.6 – 85.6
61.5 – 73.5
49.4 – 61.4
37.3 – 49.3
25.2 – 37.2
13.1 – 25.1
1.0 – 13.0
50 40 30 20 10 0 10 20 30 40
% Relative Frequency
Quadrant Isotonic Buffer Hypotonic Buffer
Live Dead Total Live Dead Total

1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4

1 42 9 544 0 2 4 42 11 548 5 12 3 0 9 400 5 21 403

2 43 5 416 0 2 3 43 7 419 3 5 6 0 13 400 3 18 406

3 41 1 528 0 3 5 41 4 533 4 2 8 0 12 400 4 14 408

4 38 2 656 0 1 6 38 3 661 5 2 1 0 15 400 5 17 401

5 47 3 368 0 2 2 47 5 370 3 3 1 2 13 400 5 16 401

Ave. No. 42. 4 502 0 2 4 42. 6 506 4 5 3 0. 12 400 4 17 404

of Cells 2 .4 7 . 4 .
8 4
Cells/mL
1,688,000
2,400,000

5,020,000

1,200,000

1,688,000

3,600,000

5,060,000

2,880,000

7,440,000

4,000,000

4,040,000
10,320,000
164,000
40,000

40,000
40,000
 Cell viability of red blood cells in varying
tonicity
4,500,000
4,000,000
3,500,000
Cells / mL

3,000,000
2,500,000
2,000,000 Live
1,500,000 Dead
1,000,000
500,000
0

Isotonic Buffer Hypotonic Buffer