that causes the disease (the “what” of the Triangle); Host (organism) harboring the disease (the “who” of the Triangle); Environment (those external factors that cause or allow disease transmission (the “where” of the Triangle) Host
Agent Envir.
Agent : is the cause of the disease.
Host : are organisms, usually humans or animals, which are exposed to and harbor a disease. Environment: the favorable surroundings and conditions external to the host that cause or allow the disease to be transmitted.
Disease-causing microbes are bacteria, virus, fungi,
and protozoa
In the center of the Triangle is time.
Time between when the host is infected and when disease symptoms occur. Or, time may describe the duration of the illness or the amount of time a person can be sick before death or recovery occurs. CAUSES OF CARIES HOST- AGENT INTERACTION • Bakteri di sulkus gingiva berinteraksi dengan sel-sel epitel ----► pertahanan awal IgA yang banyak terdapat pada sel epitel----► LPS dari bakteri yang melekat pada dinding sel epitel mengakibatkan gingiva menjadi permiabel ----► sel epitel rusak ----► fbroblast membentuk kolagen ----►bakteri mengeluarkan kolagenase ----► kolagen dirusak ----► attachment loss ----►awal proses infamasi ----►netrofl masuk ke jaringan • Interaksi LPS dengan sel epitel ----►sel epitel mensekresi IL-1β, TNF- α, IL-6 dan IL-8 • Bakteri di sulkus gingiva berinteraksi dengan sel-sel epitel ----► pertahanan awal IgA yang banyak terdapat pada sel epitel----► LPS dari bakteri yang melekat pada dinding sel epitel mengakibatkan gingiva menjadi permiabel ----► sel epitel rusak ----► fbroblast membentuk kolagen ----►bakteri mengeluarkan kolagenase ----► kolagen dirusak ----► attachment loss ----►awal proses infamasi ----►netrofl masuk ke jaringan • Interaksi LPS dengan sel epitel ----►sel epitel mensekresi IL-1β, TNF- α, IL-6 dan IL-8 CAUSES OF PERIODONTAL DESEASE
• The most common forms of human periodontal
disease are gingivitis and periodontitis. • Gingivitis is defned as an infammation of the gingiva. Gingivitis is reversible • The defnition of periodontium includes cementum, periodontal ligament, alveolar bone, and the gingiva; and periodontitis includes loss of attachment of periodontal tissues from the tooth and net loss of alveolar bone height, while regeneration after the destruction during periodontitis is not predictably achievable. - The role of dental plaque The presence of bacteria in the oral cavity has been known - The bacterial etiology of periodontal diseases has been explored for over 100 years, evolving along with technologic advances in identifcation and characterization. - Early studies indicated that periodontal diseases occurred in response to plaque mass BIOFILM - Cell–cell communication. An important characteristic seen only in bioflm associated bacteria is quorum sensing, This process is mediated by two groups of compounds known as autoinducer 1 and autoinducer 2. Gram-positive and gram- negative cells secrete molecules known as autoinducer-2 - Gene transfer. Bioflm-associated bacteria communicate with each other by way of horizontal gene transfer. In S mutans, quorum sensing is mediated by competence-stimulating peptide. Genes of the competence-stimulating peptide signaling system (comC, comD, comE, and comX) are responsible for multiple functions: bioflm formation, competence (ability to accept foreign DNA), and acid tolerance - Antimicrobial resistance. The bioflm provides a protected environment against antimicrobial agents. The bioflm acts as a barrier to diffusion due to the presence of neutralizing enzymes (b- lactamase, IgA protease) and a diffusion-resistant matrix. - Cells in the bioflm can develop antibiotic resistance from horizontal gene transfer and mutations and by expressing efflux pumps. - Thus, it has been argued that ‘‘bioflm inhibitory concentration’’ is a more realistic estimate of antimicrobial activity than the current measures [ - Regulation of gene expression. Bioflm living has been shown to regulate gene expression in certain bacteria. For example, exposure of S gordonii to saliva results in the induction of genes (sspA/B) that mediate host- surface binding and coaggregation with Porphyromonas gingivalis and Actinomyces. Similarly, genes encoding glucan (gtf) and fructan (ftf) synthesis are differentially regulated in bioflm-associated S mutans -The last decade or so has brought signifcant advances to the understanding of periodontal disease pathogenesis. -The recognition of dental plaque as a bioflm; the identifcation and characterization of genetic defects that predispose to periodontitis; the role of risk factors in disease susceptibility; and the discovery of new host-derived cellular and molecular mechanisms implicated in periodontal tissue destruction. -Many of these discoveries hold promise for the future as foundation for the engineering of new prevention and treatment modalities.