EVALUATION OF ANTI-DIABETIC

ACTIVITY OF CALYCOPHYLLUM
SPRUCEANUM
Presented By,
U.SAILEELA
Regd.No: 17FJ1S0111
Under the guidance of
Mrs.SHREYA GUPTA, M.Pharm.
Department of Pharmacology
SSJ College Of Pharmacy
 CONTENTS
 Aim and objective
 Introduction
 Literature review
 Plant profile
 Experimental models
 Parameters
 AIM AND OBJECTIVE
To study the evaluation of anti-diabetic
activity of Calycophyllum spruceanum in
animal models.
 To estimate the blood glucose levels.
 To determine the body weight of the animal.
 To examine the histo -pathological section
of pancreas.
 INTRODUCTION

Diabetes mellitus:
Diabetes mellitus is a
chronic metabolic disorder caused by
lack of insulin or reduced insulin
activity in the body which results in
hyperglycaemia.
ROLE OF PANCREAS
CLASSIFICATION OF DIABETES MELLITUS

Three types

 Type I Diabetes
 Type II Diabetes
 Gestational Diabetes
 DIABETES MELLITUS
Type I DM
 Insulin dependent diabetes
mellitus (IDDM).
 Occurs in young population.
 Also called as juvenile onset
diabetes.
Type II DM
 Non insulin dependent
diabetes mellitus (NIDDM).
 Commonly occurs in
population over 40 years of
age.
 Also called as adult onset
diabetes.
 ANTIDIABETIC-ACTIVITY
 The chemicals which are having the capacity to
reduce the increased blood glucose levels in the body
are known to posses the antidiabetic activity.

 These chemicals are screened by different methods for

their antidiabetic activity.
 LITERATURE -REVIEW
 CALYCOPHYLLUM SPRUCEANUM:

 Fabianos de vorgas (Feb 2016) Anti-oxidant activity and

peroxidase inhibition of Amazonian plants , extracts
traditionally used as anti-inflammatory.
 da silva APAB Lima Es (March 2018) Calycophyllum
spruceanum BENTH ameliorates acute inflammation in
mice.
 PLANT - PROFILE
 Plant name - Calycophyllum spruceanum
 Common name - Capirone
 Kingdom - Plantae
 Phyllum - Angiosperms
 Order - Gentianales
 Family - Rubiaceae
 Subfamily - Cinchonoideae
 Tribe - Calycophylleae
 Genus - Calycophyllum
 Species - C. spruceanum
CALYCOPHYLLUM SPRUCEANUM
Description:

 Calycophyllum spruceanum is
an evergreen tree with a tall,
narrow, open crown.
 It can grow up-to 20-30 meters.
 It is an Amazon native species.
 TRADITIONAL USES
 Eye infections
 Anti-diabetic
 Anti-oxidant
 Cosmetic
 Skin infections
 Avoids lichens, fungi, epiphytes.
 Also used for age spots, cuts, wrinkles, scars, & wounds.
 Also used to treat ovarian problems.
 EXPERIMENTAL MODELS
 Alloxan induced diabetes mellitus.
 Streptozotocin induced diabetes mellitus.
 Fructose induced diabetes mellitus.
 Dexamethasone induced diabetes.
 STREPTOZOTOCIN INDUCED DIABETES MELLITUS
Over night fasted animals

Dissolve streptozotocin (55mg/kg body weight) in 0.1M cold citrate

buffer (pH-4.5)

Inject this freshly prepared STZ solution to the overnight fasted animals
by i.p injection.

Causes diabetes mellitus

GROUPING OF ANIMALS
GROUPS TREATMENT
Group 1 Normal control (vehicle)
Group 2 Diabetic control (STZ +
Vehicle)
Group 3 Reference standard ( STZ +
Glibenclamide)
Group 4 STZ + Low dose of extract
Group 5 STZ + Intermediate dose of
extact
Group 6 STZ + High dose of extract
 Parameters
 Blood glucose levels.
 Body weight.
 Histopathological section of pancreas.
 Estimation of serum lipid profile
 Estimation of cholesterol levels
 Estimation of triglycerides
 Estimation of HDL levels
 Estimation of LDL & VLDL levels.
 DETERMINATION OF BLOOD GLUCOSE
LEVELS
 Blood samples were withdrawn from tail tip of animal.

 Blood glucose estimation can be done by one touch

electronic glucometer using glucose test strips.

 Test was repeated for 7 and 14th day of

the study.
 DETERMINATION OF BODY WEIGHT
The body weight of the animal was measured
regularly during the study by using the balance.
 HISTOPATHOLOGICAL SECTION OF
PANCREAS
 Sacrifice the animal by anesthesia.

 Pancreas was removed from each animal and collected in

10% formalin solution.

 Sections were cut and stained with hematoxylin and eosin

for histological examinations.