Beruflich Dokumente
Kultur Dokumente
Field trials.
Cleaning of glassware
bottles
Medium preparation
• Solid
• Semisolid
• Liquid
(with use of Agar Powdered)
Nutrient medium
Explant
Cell, tissue or organ of a plant that is used to start in vitro cultures
Sterilization
Aseptic environment, use of some chemicals, Hgcl2, NaOCl, Methanol
cultures
Inoculation of aseptic explant in to
nutrient medium
• Depends on our aims and objectives
Two main types
• Indirect organogenesis ( callus stage is there)
• Direct organogenesis (No callus involved)
Indirect organogenesis
Kumar et al 2016
Direct organogenesis
How this is possible?
Auxin : IAA,IBA,NAA
Cytokinin: BAP,BA,TDZ
The action of the different hormones / regulators is not consistent:
High Low
Root formation on cuttings
Adventitious root formation in callus
Callus initiation
Adventitious shoot formation
Axillary shoot growth
• Low High
Shoot proliferation and sub culturing