Presented by-

Rahima Akhter Nury: 132 1722 046

Suraiya Ahmed: 132 0916 046
Anik khan: 132 0430 046
MD Isfat Hasan Tomal: 132 0378046
Ramisha Rifat Tony: 132 0332 646
DNA Vaccine
production
What is DNA
vaccine?
• DNA vaccines are the vaccines
which contain DNA that codes for
specific proteins (antigens) from a
pathogen.
• The DNA is injected into cells
• uses the DNA to synthesize the
proteins
• Because these proteins are
recognized as foreign, when they are
processed by the host cells and
displayed on their surface, the
immune system is alerted,
• which then triggers immune
responses.
 Why DNA vaccines?
This approach offers a number of potential
advantages over traditional approaches
DNA vaccines Traditional vaccines
 Uses only the DNA from  Uses weakened or killed form
infectious organisms.
of infectious organism.
 Avoid the risk of using actual
 Create possible risk of the
infectious organism.
vaccine being fatal.

 Provide both Humoral & Cell

mediated immunity  Provide primarily Humoral
immunity
 Usually requires Refrigeration.
 Refrigeration is not required
Including :
• the stimulation of both B- and T-cell
responses,
• improved vaccine stability,
• the absence of any infectious agent
• the relative ease of large-scale manufacture.
• immune responses in animals have been
obtained using genes from a variety of
infectious agents, including influenza virus,
hepatitis B virus, human immunodeficiency
virus, rabies virus, malarial parasites etc.
• In some cases, protection from disease in
animals has also been obtained.
Principal of DNA Vaccine?

Viral gene

RecombinantDNA Expression plasmid

Technology

Plasmid with foreigngene

Transform in to bacteria

Plasmid DNA getAmplified

Plasmid DNA isolated

Stored in vials

Ready forApply
Pneumatic Jet Injection: Very high amount
of vaccine applied to the abdominal skin.
 How DNA vaccines work??
By 2 Pathway :
1. Endogenous pathway 2. Exogenous pathway
Production of DNA
vaccine
ADVANTAGES
DISADVANTAGES
APPLICATIONS
CONCLUSION
 REFERENCES
1. Wolff JA, Malone RW,Williams P,et al. Direct gene
transfer into mouse musclein
vivo. Science.1990;247(4949 Pt 1):1465–8. [PubMed]
2. Klinman DM, Yamshchikov G, Ishigatsubo Y. Contribution
of CpG motifs to the immunogenicity of DNA vaccines. J
Immunol. 1997;158(8):3635–9. [PubMed]
3. Khan KH. Vectors Used in Gene Manipulation – A
retrospective. Advanced Biotech Journal - Online-
Tutorial review. 2009;9(2):1–8
4. Corr M, Lee DJ, Carson DA, et al. Gene vaccination with
naked plasmid DNA: mechanism of CTLpriming. JExp
Med. 1996;184(4):1555–60. [PMC free article] [PubMed]
5. http://www.mdpi.com/2076-393X/1/3/225/htm