SIDDHARTH INSTITUTE OF ENGINEERING & TECHNOLOGY, PUTTUR

DEPARTMENT OF CIVIL ENGINEERINGBacterial concrete 1

TECHNICAL SEMINOR
ON
BACTERIAL CONCRETE

SUBMITTED TO: SUBIMITTED BY:

DR.G.PRABHAKARAN ME, PH.D.
PROFESSOR BHASKAR POOJITHA
DEPARTMENT OF CIVIL
15F61A0107
ENGINEERING
SIETK, PUTTUR.
 PRESENTATION PLAN

1. INTRODUCTION
2. PRINCIPLE
3. MECHANISAM
4. EVOLUTION OF CONCEPT
5. PROCEDURE
6. EXPERIMENTAL RESULTS
7. APPLICATIONS
8. LIMITATIONS
9. CONCLUSION

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 Introduction

3
•The paper presents the research done by American
scientists that tells us how bacteria can produce a cement
.
• When these bacteria are injected in sand, they form a
natural layer of cement around each individual grain.
•The fact is that microbial metabolic activities promote
calcite precipitation which acts as binding material.
•Bacillus pasteruii, a common soil bacterium can be
used to induce calcite precipitation.

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 Bacteria as self-healing agent:

4 Principle:-

• The “Bacterial Concrete” can be made by

embedding bacteria in the concrete that are able to
constantly precipitate calcite.
• Bacillus Pasteurii, a common soil bacterium, can
continuously precipitate a new highly impermeable
calcite layer over the surface of an already existing
concrete layer.
• The favorable conditions do not directly exist in a
concrete but have to be created.
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Mechanisam

As a part of metabolism, B.Pasteruii produces urease

which catalyzes urea to produce CO2 & ammonia,
resulting in an increase of pH in the surroundings ,
leading to the precipitation of (Ca32+) & (CO32-)
as CaCO3.
 Ca2+ + Cell Cell-Ca2+ . . . . (1)
 Cl- + HCO3- + NH3 NH4Cl + CO32- . . (2)
 Cell-Ca2+ + CO32- Cell-CaCO3

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 Microbiologically Induced Calcite Precipitation

6

Bacterial
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concrete
7 Evolution of concept

The idea was introduced by Ramakrishnan of South Dakota school of mines and
technology for remediating cracks and fissures in concrete structures by
employing a microbial agency.

Bacterial
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concrete
Procedure

 Primarly 12.5g of nutrient broth is added to 500ml conical flas

k contain distilled water.
 It coverd with cotton plug and is made air tight with paper and
rubber band
 It is sterilized using a cooker for about 10-20 minutes
 The solution free from contaminates and solution is clear oran
ge and addition to bacteria
 Later the flask open & extactly 1ml o the bacterium added to t
he flask at a speed of 1500-200 rpm overnight.
 After 24 hrs the solution is whitish yellow turbid solution.

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Bacterial concrete
 Applications:-

1 This technique is highly recommended for monumental & heritage structu
3
res.

 The use of bacterial concrete lead to substantial savings, especially in steel

reinforced concrete.

 It will also mean durability issues can be tackled in a new and more econo
mical way when designing concrete structures.

 Bacterial concrete is ideal for constructing underground retainers for hazar

dous waste

 For residential buildings traditional repairing of cracks will remain the mo

st economically attractive solution for now.

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Limitations

 Not more bacteria are known that can be used for calcite precipitation.
 Difficulties in the injection technology of the bacteria into sand.
 Extremely high pH of the concrete (above 12.5) hampers the use of bac
terial cell in MECR technique.
 Controlled environment is necessary.

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 CONCLUSION
 It was found that principle of microbiologically induced calcite precipitation could
be effectively used in reducing the plastic shrinkage cracks in concrete.

 Higher concentrations of bacteria had greater efficiency in remediating the plastic s

hrinkage cracks.
1
 It was found that live5 bacteria had greater efficiency in remediating the plastic shrin
kage cracks than killed bacteria.

 Bio-remediation had greater efficiency in remediating the plastic shrinkage cracks, t

han chemical remediation.

 It increases the compressive strength of treated cube specimens by 12-13% and has
remarkable potential as sealant for concrete cracks.

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 THANK YOU FOR YOUR ATTENTION…

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results