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Presented By:-
Saurabh Sharma
RY1005A08

Metwally .M.A.A. ,2009, Effect of Garlic (u

 on Some Heavy Metal
 
 
Induced Alteration in Serum Lipid Profile of 


 World Journal of Fish and Marine
Sciences 1 (1): 01-06
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1) INTRODUCTION
2) MATERIAL & METHOD
a) Chemicals
b) Experimental Fish
c) Preparation of Experimental Diets
d) Experimental Design
e) Sample Collection
f) Determination of Copper and Zinc Bioaccumulation
g) Biochemical Analysis
h) Statistical Analysis
3) RESULTS & DISCUSSION
 

1. Copper is used for different reasons in aquaculture.
2. Cause toxicity if dose is increased.
3. Zinc bound to albumin and macroglobulin in serum.
4. Zinc play a role in carbohydrate digestion.
- u
 
 play a important role on some heavy metal (Copper and
Zinc) induced alteration in Serum Lipid Profile of 
 



u
 
, commonly known as å
, is a species in the onion
belonging to family Alliaceae.

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1. Garlic oil manufactured in Germany.

2. Cooper, zinc sulphate in the present study were obtained from El. Nasr
Co., Egypt.

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1. Average body weight of 20-25 g/ fish were used in the present study.
2. Fish were apparently healthy and free from any abrasions or external
parasites.

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Six diets were prepared as shown in Table

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1. Fish were divided into 6 groups (each group has 10 fish).

2. Group 1 : control group.
3. Group 2 fed on diet with 250mg garlic oil/kg.
4. Group 3 fed on diet with 400mg zinc/kg.
5. Group 4 fed on diet with 300mg copper /kg.
6. Group 5 fed on diet with 400mg zinc/kg and 250mg garlic oil /kg.
7. Group6 fed on diet with 300mg copper/kg and 250mg garlic oil /kg as
indicated in Table.

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Ë After the end of experiment, blood and liver tissues samples were
collected from the fish.

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Ë Copper bioaccumulation was measured in blood, serum and tissues
using spectrophotometer.

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1. Serum total cholesterol was determined.

2. LDL, HDL, VLDL were assayed using an enzymatic estimation kit.

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1. Data were analyzed by Analysis of Variance(ANOVA) using the SAS

program.
2. Duncan¶s multiple range test was used to verify significance of the mean
differences among treatments.


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1. Copper/ Zinc supplemented diets compared to the control group.
2. The results showed significant (P<0.01)increase in copper
bioaccumulation in blood serum and liver in group 4.
3. Ceruloplasmine activity in blood serum showed significantly (P<0.01)
increase in fish groups4 fed on copper supplemented diet as compared to
the control group .
4. Glutathione peroxidase activity in liver tissues was significantly high in
fish groups 3 & 4 fed on diets contained copper or zinc as compared to the
control group and or garlic treated groups.
5. VLDL and LDL showed significantly (P<0.01) increase in fish groups 3&
4 fed on Cu or Zinc.
6. But HDL shows significantly (P<0.001) decrease in fish groups 3& 4 fed
on copper or Zinc.

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Both Copper and Zinc affect the serum lipid profile and garlic oil
treatment relatively improved serum lipid profile and garlic oil treatment
relatively improved serum lipid profile in treated fish.

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