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• Substrate Utilization
dS X m S X
rS
dt YX / S K S S YX / S
DPf P YP / X X 0
• If Pf = 0
YP / X X
P
D
Substrate Balance on Chemostat -
Intracellular Product
dci
VR Fcif Fci VR rfi
dt
• If ci = S
1 dS
FS0 FS VR X VR
YX / S dt
• At Steady State
X
DS 0 S X YX / S S0 S
YX / S
• With Monod
Ks D
X YX / S S0
m D
Chemostat with Extracellular
Product
• Cell Mass Balance
dX
VR FX 0 FX VR X
dt
D
• Substrate Balance
1 1 dS
FS0 FS VR X VR qP X VR
YX / S YX / P dt
F, X0 F, X2
V, X1
F+FR, X1
FR, XR
F, X0 F, X2
V, X1
F+FR, X1
Chemostat with Recycle cont.
Define Substitutions
= FR/F • F + FR = (1 + )F
recycle ratio • FRXR term
C = XR /X1 FR = F
concentration factor XR = CX1
FRXR = CFX1
dX 1
F X0 + FR XR - (F+ FR) X1 + VX1 = V
dt
dX 1
F X0 + CFX1- (1 + )F X1 + VX1 = V
dt
Recycle cont
• Assume
dX 1 Chemostat can be
– steady state =0
dt operated at higher
dilution rates than the
– sterile feed X0 = 0 specific growth rate
Then when cell recycle is
(C - 1 -)F + V = 0 used.
If D = F/V for recycle
= D(1+ (1 -C))
D YX / S ( S0 S )
X 1 YX / S ( S0 S )
(1 C )
Recycle Substrate cont.
• Assuming Monod
K S D(1 C )
S
max D(1 C )
YX / S K S D(1 C )
X1 S0
(1 C ) max D(1 C )
In Class Exercise -
• Consider a 1000 L CSTR in which biomass is
being produced with glucose as the substrate. The
microbial system follows a Monod relations with
m = 0.4 hr –1, KS = 1.5 g/L, and yield factor = 0.5
g/g. If S0 = 10g/L glucose and F = 100 L/h:
– What is the specific biomass production rate (g/l-h) at
SS?
– If recycle is used with a recycle stream of 10 L/h and a
recycle biomass concentration five times as large as that
in the reactor exit, what would be the new specific
biomass production rate?
Chemostat in Series
F, S0
V1, X1, F’, S’0
S1 F, S1, X1
V2, X2,
S2
F2, S2, X2
Chemostat in Series
(no additional feed)
• First stage (assuming Monod)
K S1 D1
S1
m1 D1
X 1 YX / S S 0 S1
• Second Stage
dX 2
FX 1 FX 2 V2 2 X 2 V2
dt
Chemostat in Series cont.
• At Steady State
X1
2 D2 1
X2
X1
1, 2 D2
X2
• Substrate Balance
1 dS
FS1 FS 2 V2 2 X 2 V2
YX / S dt
Chemostat in series
• At Steady State
2 X 2
S 2 S1
D2 YX / S
1 dS
F1S1 F ' S ( F1 F ' ) S 2 V2 2 X 2
'
0 V2
YX / S dt
• At steady state the two equations can be
solved simultaneously for S2 and V2
• Major advantage is to separate production
from growth
In Class Example – 9.2
• In a two stage chemostat system, the volumes of the
first and second reactors are 500 L and 300 L
respectively. The first reactor is used for biomass
production and the second is for a secondary
metabolite formation. The feed flow rate to the first
reactor is F = 100 L/h, and the glucose concentration is
5.0 g/L. Use the following constants for the cells.
m = 0.3 h-1, Ks = 0.1 g/L Y X/S= 0.4 g/g
• Determine the cell and glucose concentrations after the
first stage.
• Assume that growth is negligible in the second stage
and the specific rate of product formation is qP = 0.02
gP/g cell hr, and Y P/S = 0.6 gP/gS. Determine the
product and substrate concentrations in the effluent of
the second reactor.
Fed Batch Reactor
• Reactor Design Equation
V dN A
FA0 FA rA dV
dt
• No outflow FA = 0
• Good Mixing rA dV
term out of the integral
dN A d C A V
FA0 rA V
dt dt
Fed Batch Continued
• Convert the mass (NA) to concentration. Applying
integration by parts yields
dC A dV
FA0 rAV V CA
• Since dt dt
dV
FA0
• Then dt
dC A
FA0 rAV V C A FA0
• Rearranging dt
dC A FA0 C A FA0
rA
dt V V
Fed Batch Continued
• Or dC A FA0
1 C A rA
dt V
d (Vci )
Vrfi F (t )c fi
dt
dV
F (t )
dt
dS t
X t
FS0
Substrate no substrate out
(Flow out = 0)
balance dt YX / S
t
Cell dX
balance X t
dt
Fed batch cont.
• Quasi steady state for St – change in substrate
is very small in reactor and is consumed as
rapidly as fed
X t
FS0
YX / S
t
dX
X FYX / S S 0
t
Integrating from t=0 to t
dt
X X 0 FYX / S S 0t
t t
Fed batch cont.
• Quasi steady state for St – change in substrate
is very small in reactor and is consumed as
rapidly as fed (then, dSt/dt = 0)
X t
FS0
YX / S
YX / S FS0
X
t
dX t
X t FYX / SS0 Integrating from t=0 to t
dt
X X 0 FYX / SS0 t
t t
Note, "t" was missing.
Fed batch cont.
X X FYX / SS0 t
t t
0
2
• in terms of product concentration
V0 V0 Dt
P P0 q pX m t
V V 2
Class Exercise – 9.4
• Penicillin is produced in a fed-batch culture with the
intermittent addition of glucose solution to the culture
medium. The initial culture volume at quasi-steady state
is V0= 500 L, and the glucose containing nutrient
solution is added with a flow rate of F = 50 L/h. X0 = 20
g/L, S0 = 300 g/L, m = 0.2 h-1, Ks = 0.5 g/L and Y x/s=
0.3 g/g
• Determine culture volume at t = 10 h
• Determine concentration of glucose at t = 10 h
• Determine the concentration and total amount of cells at
t = 10 h
• If qp = 0.05 g product.g cells h and P0 = 0.1 g/L,
determine the product concentration at t = 10 h