A

Seminar
on
“BIO-PRINTING
IN MEDICAL USE(BIO-INK)”

By Under the guidance of

Mr. OMKAR . A . GAIKWAD Prof. Mr. D.R. Kotkar sir
EXAM SEAT NO.-T150600819

DEPARTMENT OF MECHANICAL ENGINEERING

YEAR 2018-19
  Abstract
 Graphical abstract
 Introduction
 Types
 Applications
 Summary
 Literature and review
 References

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 This technology has widely evolved around the various
bibliometric tools which are used in medical.
 “Hydrogels” and “steam cells” are the some of the most
recent fonts that are used in technology . Where the “in
vitro” is remained as the keyword.
 Number of countries and journals involved substantially
grew in one decade .
 Neither the United States’ leadership in this productivity,
nor the role of universities in publications were challenge.
 “Biomaterials” and “bio fabrications” are still the leaders of
these fields.

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 Bio-printing’ was firstly defined in 2010 as , the use of
computer aided processing for patterning and assembling
living and non living materials .
 Tissue engineering has been intended to develop practical
replacement for damaged tissues by means of applying
biology and engineering principles.
 Tissue engineering tools is one of the most promising
technologies. We have been introduced to this technology for
overcoming the organ shortage issues in medical industry. As
“bio printing”.
 Where the ‘bibliometric study’ is aimed to study the deep
evaluation of bio printing literature over past decades .

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Hydrogel Stem cell

Bio inks

In vitro

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 Bio- Bio
Cells Hydrogel printed
printer
organ

Pre-
processing

Processing
Three phases

Post-
processing
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Design and 3D printing of alginate
hydrogel structures
3Dprinted structures were designed using the CAD program Solid
Edge V20. Vascular structure design. The CAD files were then
converted to stl files and transferred to the relative software to
generate printing paths. The partially cross-linked alginate hydrogel
was loaded into the extrusion syringes and then nozzles with
0.33mm ID or 0.51mm ID (TE series Nozzles, OK International,
Hampshire, UK) fitted to the end of syringes. The printing path
width was 0.35mm and 0.55mm respectively as well as printing
height of 0.3 and 0.475 mm. The printing speed was 6mms−1
which was ideal for both set of nozzles. Extrusion speed was to be
set as 0.45 ml min−1 and 0.65 ml min−1 respectively.

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 3D Printing of Radiographic Image

It is important to note that two-dimensional (2D) radiographic images, such

as x-rays,
Magnetic resonance imaging (MRI), or computerized tomography (CT)
scans, can be converted to digital 3D print files, allowing the creation of
complex, customized anatomical and medical structures.

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 Faster and more precise than traditional methods of building organs by
hand.
 Less prone to human error.
 Less laborious for scientists.
 Organs unlikely to be rejected after transplantation.
 Reduced organ trafficking.
 Decreased waiting times for organ donors.
 Decreased animal testing.
 Finished products are independent of biomaterial or scaffolding absent in
native tissues.
 Effects of disease states or drugs may be more accurately observed without
the need for human subjects.
 Reproducibility of tissue is ensured through tight control of both
composition and geometry; reduced variability.
 Well-organized, diverse cell types allow enhancement of tissue-specific
functions.

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 Questions of liability if a printed object fails.
 Disputed ownership of the codes and implants produced.
 Various ethical concerns.
 Pricing; availability to only the wealthy.
 Consumption of large amounts of energy.
 Emission of unhealthy particles into the air.
 Difficulty in maintaining cell environment, resulting in the
death of many cells.

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 Bio inks are used to make complex tissue model by using
living cells with the help of 3d printing.
 3D bio printing is an emerging technology with various
application in making functional tissues that constructs to
reflect injured or deceased tissues.
 They deposit as filament during additive manufacturing
process.
 They are distinguished from traditional bio materials like
hydrogels polymer networks & foam scaffolds due to
deposition ability.

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 Sr. Name of Title of the Paper Research Gap Year of
No. Author publication
1. Adrien Naveau A bibliometric Study to Assess Bioprinting technology was 20 Dec 2017
et.al. Bioprinting Evolution the beginning of maturation
of Bioprinting literature .
And this products had to go
through the long journey of
healthcare market.

2. Soroosh derakhshafar 3d Bioprinting for biomedical Research on development of 25 Nov 2017

et.al devices and tissue engineering :a bio inks ,3d bioprinting
review of recent trends and methods and current state of
advances. art

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Sr. Name of Title of the Paper Research Gap Year of
No. Author publication
3. Atabak Ghanizadeh Three dimensional bio printing of New bio printing technology for 3d 22 Dec 2015
Et. Al. complex cell laden alginate hydro printing of alginate based hydro gel
gel structures and evaluated its applicability for 3d
bio printing of tumor cell

4. Xian liu et.al. Delivery of growth factors using a The physiochemical properties of 28 Jan 2014
smart porous non composite complexes through bifunctional PEG
scaffold to repair a mandibular for efficient delivery of siRNA.
bone defect

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  A bibliometric Study to Assess Bioprinting Evolution.
 3d Bioprinting for biomedical devices and tissue
engineering :A review of recent trends and advances.
 Three dimensional bio printing of complex cell laden
alginate hydrogel structures.
 Delivery of growth factors using a smart porous non
composite scaffold to repair a mandibular bone defect

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 This is newly introduced topic to study, safety
guidelines are not yet established in this field.
 The side effects of bioprinting are rarely been seen,
including questions such as biomaterials degradation
and tissue integration, biocompatibility, and continuous
tissue synthesis during material degradation.
 It takes 1,690,912,929,600hours to print a liver for
every member of the human world.

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