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ASPIRATION CYTOLOGY
( FNAC )
DEFINITION
Kidney Peritoneum
Therapeuticcyst/abcessdrainage
Face Mask
• PLUNGER: MOVABLE PORTION OF THE SYRINGE THAT HAS A RUBBER SEAL AT THE
END AND FITS INTO THE BARREL TO CREATE POSITIVE OR NEGATIVE PRESSURE BY THE
OPERATOR
• BARREL: HOLLOW PORTION OF THE SYRINGE INTO WHICH THE PLUNGER FITS
• ADAPTOR OR SYRINGE TIP: END OF THE BARREL TO WHICH THE NEEDLE HUB IS
ATTACHED
HOLLOW
BORE Shaft: hollow tubular portion of the needle
NEEDLE between the hub and the needle tip
PARTS
Tip: opposite the hub that usually is beveled
to a point and contains a lumen that is
continuous with the hollow shaft and hub
RISKS AND
CONTRAINDICATIONS
‘OVERWHELMING VOLUME OF
LITERATURE SUPPORTS THE SAFETY OF
FNA’
Minor pain / discomfort (increases
with needle size)
MOST
COMMONLY
ENCOUNTERED
Bleeding (ecchymosis / hematoma)
COMPLICATIONS
/RISKS
Severe hypoxemia
Vascular tumors
SITES OF LESIONS Pheochromocytoma
ASSOCIATED
WITH INCREASED Hepatocellular carcinoma
RISK OF
COMPLICATIONS
Pancreatic lesions
Infected tissues
BEFORE STARTING THE
PROCEDURE
Greet Greet the Patient
EVENTS studies
Explain Explain biopsy procedure in lay terms, specimen adequacy and waiting time,
obtain informed consent, and reconfirm site of aspiration
Mention Mention to the patient that several passes (averaging 2 - 6 passes) may be
necessary to obtain adequate cells for diagnosis and any other studies
Inquire Inquire about any significant medical problems including bleeding disorders,
anticoagulation, previous hisotry of syncope & Biopsy procedure Complications.
Inquire Inquire about special clinical requests (e.g. hormone studies, microbial cultures,
thyroglobulin wash out)
Examine Examine the aspiration target / site, especially location relative to the anatomical
structures
Address Address any patient concerns about the procedure BEFORE proceeding
• READY ASPIRATION SETUP AND SUPPLIES (NEEDLES,
SYRINGES, SLIDES, SPECIAL COLLECTION TUBES FOR ANY
ADDITIONAL STUDIES)
PATIENT HISTORY
(FOCUSED)
PATIENT’S HISTORY ( OPQRST )
Whether any movement, palpation, Example: "Is this the only enlarged lymph
medications or other external factor makes node you have noticed or are there more in a
lesions/symptoms better or worse different area?"
If there is pain in a lump, you can ask if it
extends or moves to any other area
Quality Severity
This is the patient's description of If there is pain you can assess with a pain score
associated symptoms, for example pain ( sharp, (usually on a scale of 0 to 10) or ask as a
dull, crushing, burning, tearing/ also the pattern comparative such as "... compared to the
intermittent, constant, throbbing ) worst pain you have ever experienced"
REQUEST FORM
• HISTORY SHOULD BE DOCUMENTED ON THE REQUEST FORM AS WELL AS
THE PHYSICAL EXAMINATION POINTS
• OTHER RELEVANT HISTORY SPECIFIC FOR THAT AREA / OR THAT IS
MENTIONED IN THE REQUEST FORM SHOULD BE NOTED SUC AS THYROID
( PALPITATIONS, TREMORS ETC ) LYMPH NODE ( FEVER ETC), BREAST.
• SOME GENERAL INFORMATIONAL TO BE DOCUMENTED IS AS FOLLOWS
1. DEMOGRAPHICS INCLUDING FACILITY NAME, PATIENT NAME, DATE
AND TIME OF EXAM, ETC.
2. RELEVANT PATIENT CLINICAL INFORMATION AND PHONE NUMBER
3. WHO PERFORMED THE FNA AND HOW MANY ATTEMPTS DONE
4. AMOUNT/COLOR/CONSISTENCY OF THE MATERIAL ASPIRATED
PHYSICAL EXAMINATION
- LUMP
(FOCUSED)
Site Size Shape Surface Skin Scar
Tenderness,
Depth Consistency Attachment Mobility
Temprature
Special collection methods needed (e.g. RPMI for flow cytometry, cell
block, sterile container for cultures, or collection / drainage of cyst
fluid)
WHEN YOU START
Follow Follow "universal precautions"
Locate and Locate and immobilize target again with one hand
immobilize
Disinfect Disinfect the skin with alcohol (70%) at site of planned needle puncture site
ALWAYS
Pass Pass the needle through the skin in one quick motion
EXCEPTIONS
EXCULDED Approach Usually needle approach is 30 - 45 degree angle to the skin for very superficial
targets and a more perpendicular approach for deep targets
Advance the needle into the Center ( Small Target ) or periphery ( Large Target –
Advance Chance of central necrosis )
In most cases, the aspirator will notice a difference
in the consistency of the tissue of the target when
penetrated
ONCE YOU Once in the target, you may apply suction, and
HAVE then the needle is moved in long back and forth
cutting motions within the target (DO NOT let the
needle come out of the skin during this motion)
ENTERED
When blood or material appears in the hub of the
needle the aspiration should be stopped
PROIR to withdrawal of the
needle if using suction,
negative pressure must be
WITHDRAW
Remove the needle from the patient by pulling
straight out so as not to lacerate the skin of the
patient by angling the needle upon withdrawal
Performing the CUTTING action with the needle tip and Limit the time of
the biopsy to less than 5 - 10 seconds
redirect the needle only when at the top of the cone (almost withdrawn
from the target) and not at the base ( Chance of laceration )
Use of long amplitude excursion and perform only 2-3 per second
Inexperience
Traditional FNA, Suction or Aspiration biopsy The French, Zajdela, Needle only or Non aspiration
Technique
Needle biopsy using suction (negative pressure) by attached Does not use suction (negative pressure) during the biopsy
syringe
DO NOT pump the syringe Relies solely on capillary action of the needle
Syringe may be directly attached to needle OR IV tubing Only the needle is used
may be placed between needle and syringe
Steady suction during the needle excursion is recommended Rotating the needle during the excursions may be helpful to
increase the yield
Traditional (suction) FNA Capillary (non aspiration technique)
May provide larger yield of cells May yield fewer cells but provides sufficient material for diagnosis
Comparatively more hemorrhage / bloody specimens Less hemorrhage / bloody specimens and higher concentration of cells
in the specimen
Less tactile sense to "feel" the consistency of the target (fingers Better tactile sense to "feel" the consistency of the target (fingers are
are farther away from the needle) closer to the needle)
Equipment may be more alarming to the patient because of the Less "scary" to the patient as this small needle only technique
addition of the large syringe and syringe holder may be concealed within your biopsy hand
Less fine motor coordination of the needle tip (uses wrist, arm and Increased fine motor coordination of the needle tip and thus
shoulders to control sampling) increased sampling precision (using fingers to control sampling);
May be more useful for very small targets
Chances of causing trauma to cells and tissues Reduces trauma to cells and tissues
Can be used to drain cystic lesions or perform therapeutic However, cannot drain cystic lesions or perform therapeutic
decompression of a target if needed decompression of a target if needed
The literature is conflicted regarding the
superiority of one technique over the
other; they may be used consecutively
during a single procedure if needed
TECHNIQUE
OF CHOICE
Personal preference and teaching
exposure will probably influence the
FNA technique of choice; however, it is
advantageous to be familiar and
proficient in both techniques
VARIABLES THAT INFLUENCE
AMOUNT OF SPECIMEN
COLLECTED INCLUDE
Needle movement (number,
Suction (force, duration and frequency and amplitude of
rate of increase) passes, angle between the
passes, acceleration of
movement)
Often create air bubbles under cover slips as they are very thick
TRANSFERRING
Make sure needle is easily removed from syringe tip BEFORE
FNA MATERIAL aspiration occurs and do not spray the material to avoid drying or
breaking of material
FROM NEEDLE
ONTO GLASS Clear everything / everyone from path between aspiration site and
SLIDE slide making site as you will be moving rapidly between those areas
with a "dirty needle"
Make smears immediately after you place material onto glass slide
(seconds count) as delaying may cause clotting and trapping of the
material.
Personal preference and teaching exposure
influence the method of choice but the physical
properties of the FNA material
TYPES OF
Express the FNA material on the slide so that it is
SMEAR about 2/3 the way up from the non frosted end of
the slide
MAKING
Generally, one to two drops forming a single large
METHODS droplet of semisolid FNA material should suffice per
slide (1 drop = ~.05 mL)
ONE STEP
A SECOND SLIDE, CALLED THE "SPREADER SLIDE", IS THEN BROUGHT UP WITH THE DOMINANT HAND BY
GRASPING IT BY THE FROSTED END PINCHED BETWEEN THE THUMB, INDEX AND THIRD FINGER TO A
POSITION THAT IS PERPENDICULAR ABOVE THE "NON SPREADER" SLIDE
SMEAR • KEEPING THIS PERPENDICULAR ORIENTATION, THE MIDDLE OF THE "SPREADER SLIDE" IS THEN POISED OVER
THE SPECIMEN DROPLET ON THE "NON SPREADER" SLIDE WITH ITS LOWER EDGE OF THE LONG AXIS
TOUCHING THE "NON SPREADER" SLIDE AND FORMING A HINGE LIKE CONTACT BETWEEN THEM
METHOD • "SPREADER SLIDE" IS THEN ROTATED FORWARD (WHILE STILL MAINTAINING THE HINGE LIKE CONTACT)
ONTO THE DROPLET WITH GENTLE PRESSURE TO FLATTEN BUT NOT CRUSH IT
• "SPREADER SLIDE" IS THEN PULLED DOWN THE SURFACE OF THE "NON SPREADER" SLIDE (WHICH IS HELD
STEADY) TO SMEAR THE DROPLET USING CONSTANT AND GENTLE PRESSURE ON THE "SPREADER SLIDE"
WHILE CONTINUING TO MAINTAIN THE PERPENDICULAR ORIENTATION OF THE TWO SLIDES
• THE "SPREADER SLIDE" SHOULD HAVE LITTLE TO NO MATERIAL ON IT AND MAY BE DISCARDED
• THE SLIDE WITH THE DROPLET OF FNA MATERIAL IS HELD UP SO IT IS ESSENTIALLY
PARALLEL WITH THE FLOOR BY USING THE NON DOMINANT HAND TO HOLD THE
FROSTED END PINCHED BETWEEN YOUR THUMB, INDEX AND THIRD FINGER
TWO STEP
INVERTED FASHION BY GRASPING IT BY THE FROSTED END PINCHED BETWEEN
THUMB, INDEX AND THIRD FINGER
• THIS INVERTED SLIDE IS THEN POISED OVER THE SPECIMEN DROPLET OF THE SLIDE
PULL BELOW IN A PARALLEL FASHION WITH THE EDGE OF THE NON FROSTED END OF THE
INVERTED SLIDE TOUCHING JUST BELOW THE FROSTED END OF THE DROPLET SLIDE
• THE INVERTED SLIDE IS THEN LOWERED (WHILE STILL MAINTAINING THE HINGE LIKE
CONTACT OF THE LOWER EDGE OF THE LONG AXIS) ONTO THE DROPLET WITH
GENTLE PRESSURE TO FLATTEN BUT NOT TO CRUSH THE DROPLET
• BOTH SLIDES ARE THEN PULLED APART TO SMEAR THE DROPLET WHILE CONTINUING
TO MAINTAIN THE PARALLEL ARRANGEMENT OF THE SLIDES USING CONSTANT AND
GENTLE PRESSURE
• BOTH SLIDE SHOULD HAVE A GOOD QUALITY OVOID SHAPE OF SMEAR MATERIAL
STAINING
• PUT THE SLIDES IN THE SLIDE CONTAINER WITH REQUIRED
MEDIUM AND TOGETHER WITH ATTACHED THE
INVESTIGATIONS ( ON THE REQUEST FORM ) SEND THE
SLIDES FOR STAINING
ULTRASOUND GUIDED
FNAC
MANY MEDICAL SUBSPECIALISTS USE ULTRASOUND (US) FOR
EVALUATION AND INTERVENTIONAL PROCEDURES BUT PATHOLOGIST
USE IS RELATIVELY NEW.
GENERAL